| Author | Melo, Elizabeth Hortêncio de | |
| Author | Gomes, Harrison Magdinier | |
| Author | Suffys, Philip Noel | |
| Author | Lopes, Márcia Quinhones Pires | |
| Author | Teixeira, Raquel Lima de Figueiredo | |
| Author | Santos, Ícaro Rodrigues dos | |
| Author | Franco, Marília Masello Junqueira | |
| Author | Langoni, Helio | |
| Author | Paes, Antonio Carlos | |
| Author | Afonso, José Augusto Bastos | |
| Author | Mendonça, Carla Lopes de | |
| Access date | 2021-11-30T14:15:09Z | |
| Available date | 2021-11-30T14:15:09Z | |
| Document date | 2021 | |
| Citation | MELO, Elizabeth Hortêncio de et al. Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis. Frontiers in Veterinary Science, v. 8, Article 747226, p. 1 - 8, Oct. 2021. | pt_BR |
| ISSN | 2299-1769 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50104 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Mycobacterium bovis | pt_BR |
| Subject in Portuguese | Tuberculose bovina | pt_BR |
| Subject in Portuguese | Gado leiteiro | pt_BR |
| Subject in Portuguese | Genotipagem | pt_BR |
| Subject in Portuguese | Patologia | pt_BR |
| Subject in Portuguese | Spoligotyping | pt_BR |
| Title | Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis | pt_BR |
| Type | Article | |
| DOI | 10.3389/fvets.2021.747226 | |
| Abstract | Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological
knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis
represents a risk to human health. This study aimed to perform the genotypic
characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy
herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent
for microbiological culture, and colonies compatible with Mycobacterium spp. were
obtained in at least one culture from 17/30 granulomas. All isolates were confirmed
to be M. bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping
characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified
genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique
profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C,
MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study
on the genetic variability of the infectious agent cause of bovine TB in Pernambuco
and demonstrates variability of strains in the state. Thus, it corroborates the importance
of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this
epidemiological tool being a determinant in the rigor of the sanitary practices of disease
control in dairy herds. | pt_BR |
| Affilliation | Universidade Federal Rural de Pernambuco. Programa de Pós-Graduação em Medicina Veterinária. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Higiene Veterinária e Saúde Pública. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural de Pernambuco. Clínica de Bovinos de Garanhuns. Garanhuns, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural de Pernambuco. Clínica de Bovinos de Garanhuns. Garanhuns, PE, Brasil. | pt_BR |
| Subject | Bovine tuberculosis | pt_BR |
| Subject | Dairy cattle | pt_BR |
| Subject | Genotyping | pt_BR |
| Subject | Mycobacterium bovis | pt_BR |
| Subject | Pathology | pt_BR |
| Subject | Spoligotyping | pt_BR |
| Subject | MIRU-VNTR | pt_BR |
