| Author | Sales, Érica Bravo | |
| Author | Fonseca Jr., Antônio Augusto | |
| Author | Gonçalves, Cristina Magalhães | |
| Author | Lage, Andrey Pereira | |
| Author | Andrade, Giovanna Ivo | |
| Author | Suffys, Philip Noel | |
| Author | Gomes, Harrison Magdinier | |
| Author | Dias, Natanael Lamas | |
| Author | Ferreira Neto, José Soares | |
| Author | Guimarães, Ana Marcia de Sá | |
| Author | Heinemann, Marcos Bryan | |
| Access date | 2021-12-01T19:58:45Z | |
| Available date | 2021-12-01T19:58:45Z | |
| Document date | 2021 | |
| Citation | SALES, Érica Bravo et al. Multispacer Sequence Typing for Mycobacterium bovis Genotyping. Frontiers in Veterinary Science, v. 8, Article 666283, p. 1 - 12, April 2021. | pt_BR |
| ISSN | 2299-1769 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50128 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Mycobactereium bovis | pt_BR |
| Subject in Portuguese | Tuberculose bovina | pt_BR |
| Subject in Portuguese | Genótipo | pt_BR |
| Subject in Portuguese | Epidemologia | pt_BR |
| Subject in Portuguese | MST | pt_BR |
| Title | Multispacer Sequence Typing for Mycobacterium bovis Genotyping | pt_BR |
| Type | Article | |
| DOI | 10.3389/fvets.2021.666283 | |
| Abstract | The molecular typing of Mycobacterium bovis, which causes bovine tuberculosis,
can be accomplished by combining different polymorphic markers, contributing to its
epidemiological investigation. Multispacer sequence typing (MST) is a sequencing-based
method that employs intergenic regions susceptible to higher mutation rates given the
low selection pressure. It has been applied to M. tuberculosis, but not to M. bovis. The
aim of this study was to evaluate a MST for M. bovis. A total of 58 strains isolated from
tissues with lesions suggestive of bovine tuberculosis, coming from cattle herds in six
Brazilian states and four standard samples of M. bovis were typified employing the MST
technique. Fourteen intergenic regions were used, and four types of genetic events were
reported: single nucleotide mutation (SNP), insertion, deletion, and tandem repeat (TR).
Seven loci were chosen for typing. Twenty-eight type sequences (ST) were identified,
indicating type sequences (ST) were identified, indicating a 92.9% HGDI (Hunter Gaston
Discriminatory Index). The data were used to analyze the evolutionary patterns of these
isolates and correlate them to phylogeographic lineages based on the formation of
clonal complexes generated from eBURST software. Later, we associated the MST with
spoligotyping technique, currently considered the gold standard for classification of M.
bovis. The results support the MST as an alternative method for genotyping of M. bovis.
The method has the advantage of sequencing and the availability of sequences analyzed
in public databases, which can be used by professionals around the world as a tool for
further analysis. This was the first study to identify the variability of isolates of M. bovis
by the MST method. | pt_BR |
| Affilliation | Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil. | pt_BR |
| Affilliation | Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil. | pt_BR |
| Affilliation | Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Laboratório Federal de Defesa Agropecuária de Minas Gerais. Pedro Leopoldo, MG, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Subject | Mycobactereium bovis | pt_BR |
| Subject | Bovine tuberculosis | pt_BR |
| Subject | Genotype | pt_BR |
| Subject | Epidemiology | pt_BR |
| Subject | MST | pt_BR |
