| Author | Paixão, Amanda R. | pt_BR |
| Author | Dias, Beatriz R. S. | pt_BR |
| Author | Palma, Luana C. | pt_BR |
| Author | Tavares, Natália M. | pt_BR |
| Author | Brodskyn, Cláudia Ida | pt_BR |
| Author | Menezes, Juliana Perrone Bezerra de | pt_BR |
| Author | Veras, Patricia Sampaio Tavares | pt_BR |
| Access date | 2021-12-03T17:25:44Z | |
| Available date | 2021-12-03T17:25:44Z | |
| Document date | 2021 | |
| Citation | PAIXÃO, Amanda R. et al. Investigating the phagocytosis of Leishmania using confocal microscopyy. Journal of Visualized Experiments, v. 172, p. 1-15, 29 July 2021. | pt_BR |
| ISSN | 1940-087X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50161 | |
| Sponsorship | INOVA-FIOCRUZ number 79700287000,
P.S.T.V. holds a grant for productivity in research from CNPq
(305235/2019-2). Plasmids were kindly provided by Mauricio
Terebiznik, University of Toronto, CA. | pt_BR |
| Language | eng | pt_BR |
| Publisher | MyJove Corporation | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Fagocitose | pt_BR |
| Subject in Portuguese | Parasitos | pt_BR |
| Subject in Portuguese | Células | pt_BR |
| Title | Investigating the phagocytosis of Leishmania using confocal microscopy | en_US |
| Type | Article | |
| DOI | 10.3791/62459 | pt_BR |
| Abstract | Phagocytosis is an orchestrated process that involves distinct steps: recognition, binding, and internalization. Professional phagocytes take up Leishmania parasites by phagocytosis, consisting of recognizing ligands on parasite surfaces by multiple host cell receptors. Binding of Leishmania to macrophage membranes occurs through complement receptor type 1 (CR1) and complement receptor type 3 (CR3) and Pattern Recognition Receptors. Lipophosphoglycan (LPG) and 63 kDa glycoprotein (gp63) are the main ligands involved in macrophage-Leishmania interactions. Following the initial recognition of parasite ligands by host cell receptors, parasites become internalized, survive, and multiply within parasitophorous vacuoles. The maturation process of Leishmania-induced vacuoles involves the acquisition of molecules from intracellular vesicles, including monomeric G protein Rab 5 and Rab 7, lysosomal associated membrane protein 1 (LAMP-1), lysosomal associated membrane protein 2 (LAMP-2), and microtubule-associated protein 1A/1B-light chain 3 (LC3). Here, we describe methods to evaluate the early events occurring during Leishmania interaction with the host cells using confocal microscopy, including (i) binding (ii) internalization, and (iii) phagosome maturation. By adding to the body of knowledge surrounding these determinants of infection outcome, we hope to improve the understanding of the pathogenesis of Leishmania infection and support the eventual search for novel chemotherapeutic targets. | en |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Parasito-Hospedeiro e Epidemiologia. Salvador, BA, Brasil / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Phagocytose | pt_BR |
| Subject | Parasites | pt_BR |
| Subject | Cells | pt_BR |
| e-ISSN | 1940-087X | pt_BR |
