Author | Salazar, Gerson Javier Torres | |
Author | Dias, Francisco Junio | |
Author | Ribeiro, Paulo Riceli Vasconcelos | |
Author | Brito, Edy Sousa de | |
Author | Canuto, Kirley Marques | |
Author | Coutinho, Henrique Douglas Melo | |
Author | Ribeiro Filho, Jaime | |
Author | Gallo, Monica | |
Author | Montesano, Domenico | |
Author | Naviglio, Daniele | |
Author | Zengin, Gokhan | |
Author | Costa, José Galberto Martins da | |
Access date | 2021-12-09T18:56:58Z | |
Available date | 2021-12-09T18:56:58Z | |
Document date | 2021 | |
Citation | SALAZAR, Gerson Javier Torres et al. Antioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model. Foods, 2021. | pt_BR |
ISSN | 2304-8158 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50261 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Antioxidantes | pt_BR |
Subject in Portuguese | Radicais livres | pt_BR |
Subject in Portuguese | Sobrecarga de ferro | pt_BR |
Subject in Portuguese | Stryphnodendron | pt_BR |
Subject in Portuguese | Taninos | pt_BR |
Title | Antioxidant Activity of Stryphnodendron rotundifolium Mart. Stem Bark Fraction in an Iron Overload Model | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/foods10112683 | |
Abstract | Stryphnodendron rotundifolium Mart., popularly known as “barbatimão”, is a plant species
traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases.
Considering the well-described antioxidant properties of this species, this study investigated the
protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds
were quantified and identified by Ultra-Performance Liquid Chromatography coupled with
quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (UPLC–ESI-qTOF-MS/MS)
in positive and negative ions mode analysis. Antioxidant activity was analyzed following the
iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The
analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg
gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents,
prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract significantly
protected 2-DR degradation induced by Fe2+ (72% protection) or OH (43% protection).
The ortho-phenanthroline test revealed Fe2+-chelating and Fe3+-reducing activities of 93% and 84%,
respectively. A preliminary toxicological analysis using Artemia salina revealed mortality below 10%,
at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions.
In conclusion, the findings of the present study indicate that Stryphnodendron rotundifolium is a source
of antioxidant compounds with the potential to be used in drug development in the context of iron
overload disorders, which remains to be further investigated in vivo. | pt_BR |
Affilliation | Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil. | pt_BR |
Affilliation | Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil. | pt_BR |
Affilliation | Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Embrapa Tropical Agroindustry. Multi-User Laboratory of Natural Products Chemistry. Fortaleza, CE, Brazil. | pt_BR |
Affilliation | Regional University of Cariri. Postgraduate Program in Biological Chemistry. Crato, CE, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil. | pt_BR |
Affilliation | University of Naples Federico II. Department of Molecular Medicine and Medical Biotechnology. Naples, Italy. | pt_BR |
Affilliation | University of Naples Federico II. Department of Pharmacy. Naples, Italy. | pt_BR |
Affilliation | University of Naples Federico II. Department of Chemical Sciences. Naples, Italy. | pt_BR |
Affilliation | Selcuk University. Department of Biology, Science Faculty. Konya, Turkey. | pt_BR |
Affilliation | Regional University of Cariri. Postgraduate Program in Ethnobiology and Nature Conservation. Crato, CE, Brazil / Regional University of Cariri. Postgraduate Program in Biological Chemistry. Crato, CE, Brazil. | pt_BR |
Subject | Antioxidant activity | pt_BR |
Subject | Free radicals | pt_BR |
Subject | Iron overload | pt_BR |
Subject | Stryphnodendron rotundifolium | pt_BR |
Subject | Tannins | pt_BR |