| Author | Abud, Ana Paula Ressetti | |
| Author | Paschoal, Ariane Caroline Campos | |
| Author | Kuligovski, Crisciele | |
| Author | Caruso, Rodrigo Rêgo Barros | |
| Author | Dallagiovanna, Bruno | |
| Author | Aguiar, Alessandra Melo de | |
| Access date | 2021-12-14T13:28:59Z | |
| Available date | 2021-12-14T13:28:59Z | |
| Document date | 2021 | |
| Citation | ABUD, Ana Paula Ressetti et al. Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction. MethodsX, v.8, n. 101515, p. 1–22, 2021. | pt_BR |
| ISSN | 2215-0161 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50405 | |
| Language | por | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.mex.2021.101515 | |
| Abstract | In vitro stem cell models are used as alternatives to animal models and are important tools for cytotoxicity studies. Researchers can determine the effects of test substances on human cells by evaluating cell viability and differentiation. Here, we describe an in vitro model to quantify adipogenesis based on the Nile red staining of specific lipid droplets and the emission of basic lipids from human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) in the presence of test substances. This assay allows for the prediction of toxicity based on the inhibition of adipogenesis in vitro in a 96-well format. The differentiation of a progenitor cell into a specialized cell, the adipocyte, is easy to monitor and quantify, making this a simple assay. The fluorescence staining of nuclei and lipid droplets is measured after 14 days of cell differentiation to determine cell number and assess cell differentiation using high-content imaging analysis, thus allowing for the identification of chemicals that impact differentiation. We also describe a protocol to assess adipocyte differentiation by fluorescence intensity using a multiplate reader. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. / Grupo Boticário. Pesquisa and Desenvolvimento. São José dos Pinhais, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
| Subject | Stem Cells | pt_BR |
| Subject | Cell Differentiation | pt_BR |
| Subject | Cytotoxicity | pt_BR |
| Subject | Adipogenesis | pt_BR |
| Subject in Spanish | Células Madre | pt_BR |
| Subject in Spanish | Diferenciación Celular | pt_BR |
| Subject in Spanish | Citotoxicidad | pt_BR |
| Subject in Spanish | Adipogénesis | pt_BR |
| Subject in French | Cellules souches | pt_BR |
| Subject in French | Différenciation cellulaire | pt_BR |
| Subject in French | Cytotoxicité | pt_BR |
| Subject in French | Adipogenèse | pt_BR |
| DeCS | Células-Tronco | pt_BR |
| DeCS | Diferenciação Celular | pt_BR |
| DeCS | Citotoxicidade | pt_BR |
| DeCS | Adipogenia | pt_BR |
