| Author | Melo, Caroline Vilas Boas de | |
| Author | Torres, Felipe Guimarães | |
| Author | Hermida, Micely D’El-Rei | |
| Author | Fontes, Jonathan L. M. | |
| Author | Mesquita, Bianca Ramos | |
| Author | Brito, Reginaldo | |
| Author | Ramos, Pablo Ivan P. | |
| Author | Fernandes, Gabriel R. | |
| Author | Freitas, Luiz Antônio Rodrigues | |
| Author | Khouri, Antonio Ricardo | |
| Author | Costa, Carlos Henrique Nery | |
| Author | dos-Santos, Washington Luis Conrado | |
| Access date | 2021-12-14T18:22:02Z | |
| Available date | 2021-12-14T18:22:02Z | |
| Document date | 2021 | |
| Citation | MELO, Caroline Vilas Boas de et al. Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest. Frontiers in Immunology, 2021. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50425 | |
| Sponsorship | Conselho Nacional deDesenvolvimento Cientı́fico e Tecnológico –
CNPq (grant no. 400905/2013-2) and the research productivity
scholarship provided to WS. We are also thankful for financial
support from the Coordenação de Aperfeiçoamento de Pessoal de
Nı́vel Superior – CAPES (grant no. 2072_2013) and the Fundação
de Amparo à Pesquisa do Estado da Bahia – FAPESB
(no. PET0053_2013). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmaniose visceral | pt_BR |
| Subject in Portuguese | Leucócitos | pt_BR |
| Subject in Portuguese | Baço | pt_BR |
| Subject in Portuguese | RNA-Seq | pt_BR |
| Subject in Portuguese | Baço patologia | pt_BR |
| Subject in Portuguese | Hiperplasia | pt_BR |
| Title | Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest | pt_BR |
| Type | Article | pt_BR |
| Abstract | Structural changes in the spleen have been reported in several infectious diseases. In
visceral leishmaniasis (VL), a severe parasitic disease caused by Leishmania spp., the loss
of white pulp accompanies a severe clinical presentation. Hamster model reproduces
aspects of human VL progression. In the early stages, a transcriptomic signature of
leukocyte recruitment was associated with white pulp hyperplasia. Subsequently,
impaired leukocyte chemotaxis with loss of T lymphocytes in the periarteriolar lymphoid
sheath occurred. This differential gene expression was subsequently corroborated by
transcriptomic profiling of spleens in severe human VL. At the latest stage, spleen
disorganization was associated with increasing clinical signs of VL. White pulp
disruption was accompanied by decreased DLK1 expression. The expression of
CXCL13, CCR5, CCL19, CCR6, CCR7 and LTA decreased, likely regulated by
CDKN2A overexpression. Our findings enlighten a pathway implying cell cycle arrest
and decreased gene expression involved in spleen organization. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Enfermidades Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Centro de Integração de Dados e Conhecimento para Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene´ Rachou. Informática de Biossistemas. Minas Gerais, BH, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Enfermidades Infecciosas Transmitidas por Vetores. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Piauí. Instituto de Doenças Tropicais Natan Portella. Departamento de Medicina Comunitária. Teresina, PI, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil. | pt_BR |
| Subject | Visceral leishmanaisis | pt_BR |
| Subject | White pulp remodeling | pt_BR |
| Subject | Spleen disorganization | pt_BR |
| Subject | Transcriptomic (RNA-Seq) | pt_BR |
| Subject | Spleen pathology, hamster | pt_BR |
| e-ISSN | 10.3389/fimmu.2021.716314 | |
