| Author | Reis, Eneida Virginia | |
| Author | Pereira, Roberta Verciano | |
| Author | Gomes, Matheus | |
| Author | Passos, Liana Konovaloff Jannotti | |
| Author | Baba, Elio Hideo | |
| Author | Coelho, Paulo Marcos Zech | |
| Author | Mattos, Ana Carolina Alves de | |
| Author | Couto, Flávia Fernanda Bubula | |
| Author | Borges, William Castro | |
| Author | Sá, Renata Guerra | |
| Access date | 2021-12-30T15:29:10Z | |
| Available date | 2021-12-30T15:29:10Z | |
| Document date | 2014 | |
| Citation | REIS, Eneida Virginia et al. Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni. Parasitology International, v. 63, n. 1, p. 120-126, 2014 | pt_BR |
| ISSN | 1383-5769 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50585 | |
| Language | eng | pt_BR |
| Publisher | Elsevier B.V. | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.parint.2013.10.005 | |
| Abstract | Vaults are ribonucleoproteins (13 MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The exact function of this complex is unknown, although the biological role of vaults has been associated with multidrug resistance phenotypes and signal transduction pathways. Genomic analysis showed that model organisms, such as Caenorhabditis elegans and Drosophila melanogaster, do not possess genes encoding vaults. However, we have found that vault-related genes are present in the Schistosoma mansoni genome. These observations raised questions on the involvement of vaults in mechanisms of adaptation of the parasite in its mammalian host Therefore, molecular characterisation of the putative Major Vault Protein performed using bioinformatics tools showed that this vault component is highly conserved in S. mansoni. The MVP expression level was quantified by qRT-PCR using total RNA from susceptible (LE) and resistant (LE-PZQ) adult worm lineages, cercariae and mechanically transformed schistosomula (MTS) cultured for 3.5, 24, 48 and 72 h in vitro. Our results suggest a stage-specific expression in all developmental stages analysed. Western blotting has shown up-regulation of SmMVP in the MTS-3.5, 72 h and resistant adult worms, and similar levels in all other stages. Furthermore, SmMVP was found differentially expressed in adult males and females from the susceptible lineage. Further studies should clarify whether SmMVP is somehow linked to drug resistance in S. mansoni. (C) 2013 Elsevier Ireland Ltd. All rights reserved | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Patos de Minas, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Departamento de Ciências Biológicas. Ouro Preto, MG, Brazil | pt_BR |
| Subject | Vault complex | pt_BR |
| Subject | Resistance | pt_BR |
| Subject | MVP protein | pt_BR |
| Subject | Differential expression | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
