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AutorRocha, Isadora Filipaki Munhoz da
AutorToledo, Martins, Sharon de
AutorAmatuzzi, Rafaela Ferreira
AutorZamith-Miranda, Daniel
AutorNosanchuk, Joshua D.
AutorRodrigues, Marcio Lourenço
AutorAlves, Lysangela Ronalte
Fecha de acceso2022-01-20T14:34:27Z
Fecha de disponibilización2022-01-20T14:34:27Z
Fecha de publicación2021
ReferenciaROCHA, Isadora Filipaki Munhoz da et al. Cellular and extracellular vesicle RNA analysis in the global threat fungus Candida auris. Veterinary World, p. 2735–2738, 2021.pt_BR
ISSN2165-0497pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/50833
Idiomaporpt_BR
EditorAmerican Society for Microbiologypt_BR
Derechos de autoropen accesspt_BR
TítuloCellular and Extracellular vesicle RNA analysis in the global threat fungus Candida aurispt_BR
Tipo del documentoArticlept_BR
DOI10.1128/Spectrum.01538-21
Resumen en InglésEmerging and reemerging pathogens are a worldwide concern, and it is predicted that these microbes will cause severe outbreaks. Candida auris affects people with weakened immune systems, particularly those who are hospitalized or are in health care facilities. Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all domains of life. EVs can deliver functional molecules to target cells, including proteins and nucleic acids, especially RNA molecules. EVs from several pathogenic fungi species play diverse biological roles related to cell-cell communication and pathogenhost interaction. In this study, we describe a data set which we produced by sequencing the RNA content of EVs from C. auris under normal growth conditions and in the presence of the antifungal caspofungin, a first-line drug to treat this fungus. To generate a more complete data set for future comparative studies, we also sequenced the RNA cellular content of EVs under the same conditions. This data set addresses a previously unexplored area of fungal biology regarding cellular small RNA and EV RNA. Our data will provide a molecular basis for the study of the aspects associated with antifungal treatment, gene expression response, and EV composition in C. auris. These data will also allow the exploration of small RNA content in the fungal kingdom and might serve as an informative basis for studies on the mechanisms by which molecules are directed to fungal EVs. Candida auris, a relevant emerging human-pathogenic yeast, is the first fungus to be called a global public health threat by the WHO. This is because of its rapid spread on all inhabited continents, together with its extremely high frequency of drug and multidrug resistance. In our study, we generated a large data set for 3 distinct strains of C. auris and obtained cellular small RNA fraction as well as extracellular vesicle RNA (EV-RNA) during normal growth conditions and after treatment with caspofungin, the first-line drug used to treat C. auris infection.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.pt_BR
AfiliaciónDepartment of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA.pt_BR
AfiliaciónDepartment of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.pt_BR
Palavras clave en InglêsExtracellular Vesiclespt_BR
Palavras claveVesículas Extracelularespt_BR
Palavras claveMicroARNspt_BR
Palavras clave en FrancêsVésicules extracellulairespt_BR
Palavras clave en FrancêsmicroARNpt_BR
DeCSCandida aurispt_BR
DeCSRNA-Seqpt_BR
DeCSVesículas Extracelularespt_BR
DeCSMicroRNAspt_BR


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