| Author | Azevedo, C. T. | |
| Author | Cotias, A. C. | |
| Author | Arantes, A. C. S. | |
| Author | Ferreira, T. P. T. | |
| Author | Martins, M. A. | |
| Author | Olsen, P. C. | |
| Access date | 2022-01-24T18:50:41Z | |
| Available date | 2022-01-24T18:50:41Z | |
| Document date | 2021 | |
| Citation | AZEVEDO, C. T. et al. Assessment of Allergen-Responsive Regulatory T Cells in Experimental Asthma Induced in Different Mouse Strains. Mediators of Inflammation, v. 2021, Article ID7584483, p. 1 - 12, 2021. | pt_BR |
| ISSN | 1466-1861 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50866 | |
| Language | eng | pt_BR |
| Publisher | Hindawi | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Avaliação | pt_BR |
| Subject in Portuguese | Células T reguladoras responsivas a alérgenos | pt_BR |
| Subject in Portuguese | Asma experimental | pt_BR |
| Subject in Portuguese | Indução | pt_BR |
| Subject in Portuguese | Diferentes linhagens de camundongos | pt_BR |
| Title | Assessment of Allergen-Responsive Regulatory T Cells in Experimental Asthma Induced in Different Mouse Strains | pt_BR |
| Type | Article | |
| DOI | 10.1155/2021/7584483 | |
| Abstract | Regulatory T cells (Tregs) are important in regulating responses to innocuous antigens, such as allergens, by
controlling the Th2 response, a mechanism that appears to be compromised in atopic asthmatic individuals. Different isogenic
mouse strains also have distinct immunological responses and susceptibility to the experimental protocols used to develop lung
allergic inflammation. In this work, we investigated the differences in the frequency of Treg cell subtypes among A/J, BALB/c,
and C57BL/6, under normal conditions and following induction of allergic asthma with ovalbumin (OVA). Methods.
Subcutaneous sensitization followed by 4 consecutive intranasal OVA challenges induced asthma characteristic changes such as
airway hyperreactivity, inflammation, and production of Th2 cytokines (IL-4, IL-13, IL-5, and IL-33) in the lungs of only A/J
and BALB/c but not C57BL/6 strain and evaluated by invasive whole-body plethysmography, flow cytometry, and ELISA,
respectively. Results. A/J strain naturally showed a higher frequency of CD4+IL-10+ T cells in the lungs of naïve mice compared
to the other strains, accompanied by higher frequencies of CD4+IL-4+ T cells. C57BL/6 mice did not develop lung inflammation
and presented higher frequency of CD4+CD25+Foxp3+ Treg cells in the bronchoalveolar lavage fluid (BALF) after the allergen
challenge. In in vitro settings, allergen-specific stimulation of mediastinal LN (mLN) cells from OVA-challenged animals
induced higher frequency of CD4+IL-10+ Treg cells from A/J strain and CD4+CD25+Foxp3+ from C57BL/6. Conclusions.
The observed differences in the frequencies of Treg cell subtypes associated with the susceptibility of the animals to
experimental asthma suggest that CD4+CD25+Foxp3+ and IL-10-producing CD4+ Treg cells may play different roles in
asthma control. Similar to asthmatic individuals, the lack of an efficient regulatory response and susceptibility to the
development of experimental asthma in A/J mice further suggests that this strain could be preferably chosen in
experimental models of allergic asthma. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Estudos em Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Estudos em Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Assessment | pt_BR |
| Subject | Allergen-Responsive Regulatory T Cells | pt_BR |
| Subject | Experimental Asthma | pt_BR |
| Subject | Induced | pt_BR |
| Subject | Different Mouse Strains | pt_BR |
