| Author | Marques, Barbara Bruno Fagundes | |
| Author | Guimarães, Taísa Coelho | |
| Author | Fischer, Ricardo Guimarães | |
| Author | Tinoco, Justine Monteiro Monnerat | |
| Author | Pires, Fábio Ramoa | |
| Author | Lima Junior, Josué da Costa | |
| Author | Stevens, Roy H. | |
| Author | Tinoco, Eduardo Muniz Barretto | |
| Access date | 2022-01-25T18:17:20Z | |
| Available date | 2022-01-25T18:17:20Z | |
| Document date | 2021 | |
| Citation | MARQUES, Barbara Bruno Fagundes et al. Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study. Oral Diseases, 00, p. 1 - 6, July 2021. | pt_BR |
| ISSN | 1601-0825 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50882 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Células epiteliais | pt_BR |
| Subject in Portuguese | SARS-CoV- 2 | pt_BR |
| Subject in Portuguese | Proteína de pico | pt_BR |
| Subject in Portuguese | RT-PCR | pt_BR |
| Title | Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study | pt_BR |
| Type | Article | |
| DOI | 10.1111/odi.13988 | |
| Abstract | Objective: The aim of the present case–control
study was to evaluate the morphological
aspects of the epithelial cells from the dorsum of the tongue and the expression of
the SARS-CoV-
2
Spike protein in these cells, in patients with and without COVID-19
infection.
Methods: 24 individuals with at least one symptom of COVID-19
were recruited
among inpatients from Hospital Universitário Pedro Ernesto (Rio de Janeiro, Brazil).
14 patients who tested positive for COVID-19
by RT-PCR
were included in the case
group, and 10 patients who tested negative were included in the control group.
Cytological smears from the dorsum of the tongue were obtained from all patients
and analyzed using immunohistochemistry directed against SARS-CoV-
2-
Spike
protein.
Morphological changes in epithelial cells were analyzed using light microscopy.
Results: Immunohistochemistry showed that 71% of the COVID-19
patients presented
epithelial cells positive for the presence of the SARS-CoV-
2
Spike protein, and
all cells coming from patients in the control group were negative. Cytological analysis
showed significant differences when comparing epithelial cells from COVID-19-
positive
and COVID-19-
negative
patients. Conclusion: COVID-19
may generate dimensional changes in tongue epithelial cells;
however, further studies are necessary to understand how this happens. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Odontologia. Departamento de Clínica Dental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estácio de Sá. Escola de Odontologia. Patologia Oral. Rio de Janeiro, RJ, Brasil / Universidade Estácio de Sá. Programa de Pós-Graduação em Odontologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Estácio de Sá. Programa de Pós-Graduação em Odontologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Laboratory of Oral Infectious Diseases, Maurice Kornberg School of Dentistry. Temple University, Philadelphia, PA, USA. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Epithelial cells | pt_BR |
| Subject | RT-PCR | pt_BR |
| Subject | SARS-CoV- 2 | pt_BR |
| Subject | Spike protein | pt_BR |
| DeCS | Reação em Cadeia da Polimerase Via Transcriptase Reversa | pt_BR |
