| Author | Vilela, Márcia Carvalho | |
| Author | Mansur, Daniel Santos | |
| Author | Queiroz, Norinne Lacerda | |
| Author | Rodrigues, David Henrique | |
| Author | Arantes, Rosa Maria Esteves | |
| Author | Kroon, Erna Geessien | |
| Author | Campos, Marco Antônio | |
| Author | Teixeira, Mauro Martins | |
| Author | Teixeira, Antônio Lúcio | |
| Access date | 2022-01-25T18:40:15Z | |
| Available date | 2022-01-25T18:40:15Z | |
| Document date | 2008 | |
| Citation | VILELA, Márcia Carvalho et al. Traffic of leukocytes in the central nervous system is associated with chemokine up-regulation in a severe model of herpes simplex encephalitis: An intravital microscopy study. Neuroscience Letters, v. 445, n. 1, p. 18-22, 2008. doi.org/10.1016/j.neulet.2008.08.072 | pt_BR |
| ISSN | 0304-3940 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50883 | |
| Sponsorship | FINANCIADORA DE ESTUDOS E PROJETOS - FINEP | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Traffic of leukocytes in the central nervous system is associated with chemokine up-regulation in a severe model of herpes simplex encephalitis: An intravital microscopy study | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.neulet.2008.08.072 | |
| Abstract | Herpes simplex virus type I (HSV-1) is a human pathogen that may cause severe encephalitis. The development of experimental models of HSV-1 encephalitis is relevant for the comprehension of the immune mechanisms involved in this infection. C57BL/6 mice were inoculated intracranially with 104 PFU of neurotropic HSV-1. All animals developed signs of encephalitis and died until day 6 post-infection (pi). Using intravital microscopy, we demonstrated increased leukocyte rolling and adhesion in the brain Microvasculature of infected mice at days 1, 3 and 5 pi. The infection was followed by a significant increase in chemokine levels, including CCL2, CCL3, CCL5, CXCL1 and CXCL9. TNF-alpha also showed a significant increase at day 3 pi. Histological analyses demonstrated diffuse meningoencephalitis characterized mainly by mononuclear cell infiltrates. The present model of HSV-1 encephalitis exhibits high mortality in the very first days of infection. Accordingly, there were increased rolling and adhesion of leukocytes along the brain endothelium wall and a high expression of chemokines in the central nervous system. These results corroborate the role of chemokines in leukocyte recruitment following HSV-1 infection in the central nervous system. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. . Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Patologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Microbiologia. . Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Herpes simplex virus-1 | pt_BR |
| Subject | Encephalitis | pt_BR |
| Subject | Intravital microscopy | pt_BR |
| Subject | Chemokines | pt_BR |
