| Author | Macena, Lorena da Graça Pedrosa de | |
| Author | Vieira, Carmen Baur | |
| Author | Maranhão, Adriana Gonçalves | |
| Author | Ferreira, Fernando César | |
| Author | Lemos, Elba Regina Sampaio | |
| Author | Miagostovich, Marize Pereira | |
| Access date | 2022-01-25T19:38:44Z | |
| Available date | 2022-01-25T19:38:44Z | |
| Document date | 2021 | |
| Citation | MACENA, Lorena da Graça Pedrosa de et al. Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples. Int. J. Plant. Anim. Environ Sci, v. 11, n. 4, p. 614-626, Nov. 2021. | pt_BR |
| ISSN | 2231-4490 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50886 | |
| Language | eng | pt_BR |
| Publisher | Fortune Journals | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Diversidade genética | pt_BR |
| Subject in Portuguese | HAdV | pt_BR |
| Subject in Portuguese | JCPyV | pt_BR |
| Subject in Portuguese | Monitoramento baseado em águas residuais | pt_BR |
| Title | Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples | pt_BR |
| Type | Article | |
| DOI | 10.26502/ijpaes.202125 | |
| Abstract | Wastewater-based monitoring has been described as a
non-invasive approach to assess virus distribution in a
specific geographic area. In this study we assess the
genetic diversity and concentration of human
adenovirus (HAdV) and human polyomavirus JC
(JCPyV) in wastewater samples in Rio de Janeiro, Brazil, during the Olympic Games, Rio 2016.
Wastewater samples (50 mL) obtained from domestic
and hospital sewages were concentrated by the
skimmed milk flocculation method and processed
using molecular tools. Quantitative polymerase chain
reaction using the ABI PRISM 7500 Real Time
TaqMan System and TaqMan Universal Master Mix II detected 18 HAdV and 17 JCPyV strains in 95% of
those samples (18/19). A mean viral load of 8.6x105
genomic copies (GC)/L and 1.2x107 GC/L, was
achieved for HAdV and JCPyV, respectively. Partial
nucleotide sequencing using Sanger methodology
revealed three HAdV species/ eight serotypes (HAdV
B, D, D10, D17, D19, D22, F40 and F41) and seven
genotypes/nine subtypes (JCPyV-1B, 2A, 3A, 3B, 4,
6, 7A, 8A and 8B). HAdV-D17 and -D22, as well as
genotypes JCPyV-7 and -8, were detected for the first
time in the country. The detection of previously
undetected viruses in the region demonstrates the
importance of our findings, adding data to the
epidemiology of those viruses and corroborating the
importance of environmental surveillance carried out
from wastewater. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Genetic diversity | pt_BR |
| Subject | HAdV | pt_BR |
| Subject | JCPyV | pt_BR |
| Subject | Wastewater-based monitoring | pt_BR |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
