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AutorCantanhêde, Lilian Motta
AutorMattos, Cristiane Batista
AutorCruz, Ana Karoline
AutorIkenohuchi, Yoda Janaina
AutorFernandes, Flavia Gonçalves
AutorMedeiros, Enmanuella Helga Ratier Terceiro
AutorSilva Júnior, Cipriano Ferreira da
AutorCupolillo, Elisa
AutorFerreira, Gabriel Eduardo Melim
AutorFerreira, Ricardo de Godoi Mattos
Fecha de acceso2022-01-26T14:22:18Z
Fecha de disponibilización2022-01-26T14:22:18Z
Fecha de publicación2021
ReferenciaCANTHANHÊDE, Lilian Motta et al. Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis. Pathogens, v. 10, 1116, p. 1 - 16, Sept. 2021.pt_BR
ISSN2076-0817pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/50903
Idiomaengpt_BR
EditorMDPIpt_BR
Derechos de autoropen access
Palabras clave en PortuguésLeishmaniose Tegumentarpt_BR
Palabras clave en PortuguésLeishmaniose mucosapt_BR
Palabras clave en PortuguésDiagnóstico molecularpt_BR
TítuloOvercoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasispt_BR
Tipo del documentoArticle
DOI10.3390/ pathogens10091116
Resumen en InglésThe northern region of Brazil, which has the largest number of cases of tegumentary leishmaniasis (TL) in the country, is also the region that has the highest diversity of species of vectors and Leishmania parasites. In this region, cases of mucosal leishmaniasis (ML), a clinical form of TL, exceed the national average of cases, reaching up to 12% of the total annual TL notifications. ML is associated with multiple factors, such as the parasite species and the viral endosymbiont Leishmania RNA virus 1 (LRV1). Being a chronic parasitological disease, laboratory diagnosis of ML poses a challenge for health services. Here, we evaluated more than 700 clinical samples from patients with clinical suspicion of TL, including patients with cutaneous leishmaniasis (CL) and mucosal leishmaniasis, comparing the results of parasitological tests—direct parasitological examination by microscopy (DP) and conventional PCR (cPCR) targeting of both kDNA and hsp70. The DP was performed by collecting material from lesions through biopsies (mucosal lesions) or scarification (cutaneous lesions); for PCR, a cervical brush was used for sample collection. Blood samples were tested employing standardized real-time PCR (qPCR) protocol targeting the HSP70 gene. PCR tests showed higher sensitivity than DP for both CL and ML samples. Considering ML samples only (N = 89), DP showed a sensitivity of 49.4% (N = 44) against 98.8% (N = 88) for kDNA PCR. The qPCR hsp70 for blood samples from patients with ML (N = 14) resulted in superior sensitivity (50%; N = 7) compared to DP (21.4%; N = 3) for samples from the same patients. Our results reinforced the need to implement a molecular test for the diagnosis of ML, in addition to proposing methods less invasive for collecting material from TL patients. Sample collection using a cervical brush in lesions observed in CL and ML patients is easy to perform and less invasive, compared to scarification and biopsies. Blood samples could be a good source for qPCR diagnosis for ML patients. Thus, we propose here a standardized method for collection and for performing of molecular diagnosis of clinical samples from suspicious ML patients that can be applied in reference services for improving ML diagnosis.pt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasilpt_BR
Palavras clave en InglêsTegumentary leishmaniasispt_BR
Palavras clave en InglêsMucosal leishmaniasispt_BR
Palavras clave en InglêsMolecular diagnosispt_BR


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