| Author | Fontes, Sílvia da Silva | |
| Author | Maia, Fernanda de Moraes | |
| Author | Ataides, Laura Santa’Anna | |
| Author | Conte, Fernando Paiva | |
| Author | Lima Junior, Josué da Costa | |
| Author | Rozental, Tatiana | |
| Author | Assis, Matheus Ribeiro da Silva | |
| Author | Pessoa Júnior, Adonai Alvino | |
| Author | Fernandes, Jorlan | |
| Author | Lemos, Elba Regina Sampaio de | |
| Author | Silva, Rodrigo Nunes Rodrigues da | |
| Access date | 2022-01-27T17:54:48Z | |
| Available date | 2022-01-27T17:54:48Z | |
| Document date | 2021 | |
| Citation | FONTES, Sílvia da Silva et al. Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections. Pathogens, v. 10, 1250, p. 1 - 18, Sept. 2021. | pt_BR |
| ISSN | 2076-0817 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50919 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Febre Q | pt_BR |
| Subject in Portuguese | Imunoinformática | pt_BR |
| Subject in Portuguese | Epítopo de célula B | pt_BR |
| Subject in Portuguese | Sorodiagnóstico | pt_BR |
| Subject in Portuguese | Peptídeos sintéticos | pt_BR |
| Title | Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections | pt_BR |
| Type | Article | |
| DOI | 10.3390/pathogens10101250 | |
| Abstract | Coxiella burnetii is a global, highly infectious intracellular bacterium, able to infect a wide
range of hosts and to persist for months in the environment. It is the etiological agent of Q fever—
a zoonosis of global priority. Currently, there are no national surveillance data on C. burnetii’s
seroprevalence for any South American country, reinforcing the necessity of developing novel and
inexpensive serological tools to monitor the prevalence of infections among humans and animals—
especially cattle, goats, and sheep. In this study, we used immunoinformatics and computational
biology tools to predict specific linear B-cell epitopes in three C. burnetii outer membrane proteins:
OMP-H (CBU_0612), Com-1 (CBU_1910), and OMP-P1 (CBU_0311). Furthermore, predicted epitopes
were tested by ELISA, as synthetic peptides, against samples of patients reactive to C. burnetii in
indirect immunofluorescence assay, in order to evaluate their natural immunogenicity. In this way,
two linear B-cell epitopes were identified in each studied protein (OMP-H(51–59), OMP-H(91–106),
Com-1(57–76), Com-1(191–206), OMP-P1(197–209), and OMP-P1(215–227)); all of them were confirmed as
naturally immunogenic by the presence of specific antibodies in 77% of studied patients against
at least one of the identified epitopes. Remarkably, a higher frequency of endocarditis cases was
observed among patients who presented an intense humoral response to OMP-H and Com-1 epitopes.
These data confirm that immunoinformatics applied to the identification of specific B-cell epitopes
can be an effective strategy to improve and accelerate the development of surveillance tools against
neglected diseases. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Projeto de Implantação de Planta Piloto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Q fever | pt_BR |
| Subject | Immunoinformatics | pt_BR |
| Subject | B-cell epitope | pt_BR |
| Subject | Serodiagnosis | pt_BR |
| Subject | Synthetic peptides | pt_BR |
