| Author | Girão, Luciana F. C. | |
| Author | Cavalheiro, Manuela Colla | |
| Author | Silva, Margarida Ferreira | |
| Author | Rocha, Surza L. G. da | |
| Author | Perales, Jonas | |
| Author | Martins, M. Bárbara F. | |
| Author | Ferrara, Maria Antonieta | |
| Author | Bon, Elba P. S. | |
| Author | Corvo, M. Luísa | |
| Access date | 2022-01-28T13:50:59Z | |
| Available date | 2022-01-28T13:50:59Z | |
| Document date | 2021 | |
| Citation | GIRÃO, Luciana F. C. et al. ASP-Enzymosomes with Saccharomyces cerevisiae Asparaginase II Expressed in Pichia pastoris: Formulation Design and In Vitro Studies of a Potential Antileukemic Drug. International Journal of Molecular Sciences, v. 22, 11120, p. 1 - 13, Oct. 2021. | pt_BR |
| ISSN | 1661-6596 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50930 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Asparaginase de levedura | pt_BR |
| Subject in Portuguese | gene ASP3 | pt_BR |
| Subject in Portuguese | ASP-enzimossomas | pt_BR |
| Subject in Portuguese | Nanoformulações | pt_BR |
| Subject in Portuguese | Terapia enzimática | pt_BR |
| Subject in Portuguese | Tratamento de leucemia | pt_BR |
| Title | ASP-Enzymosomes with Saccharomyces cerevisiae Asparaginase II Expressed in Pichia pastoris: Formulation Design and In Vitro Studies of a Potential Antileukemic Drug | pt_BR |
| Type | Article | |
| DOI | 10.3390/ ijms222011120 | |
| Abstract | The bacterial enzyme asparaginase is the main treatment option for acute lymphoblastic
leukemia. However, it causes side effects, such as immunological reactions, and presents undesirable
glutaminase activity. As an alternative, we have been studying asparaginase II from Saccharomyces
cerevisiae, coded by ASP3 gene, which was cloned and expressed in Pichia pastoris. The recombinant
asparaginase (ASP) presented antileukemic activity and a glutaminase activity 100 times lower in
comparison to its asparaginase activity. In this work, we describe the development of a delivery
system for ASP via its covalent attachment to functionalized polyethylene glycol (PEG) polymer
chains in the outer surface of liposomes (ASP-enzymosomes). This new delivery system demonstrated
antiproliferative activity against K562 (chronic myeloid leukemia) and Jurkat (acute lymphocytic
leukemia) cell lines similar to that of ASP. The antiproliferative response of the ASP-enzymosomes
against the Jurkat cells suggests equivalence to that of the free Escherichia coli commercial asparaginase
(Aginasa®). Moreover, the ASP-enzymosomes were stable at 4 C with no significant loss of activity
within 4 days and retained 82% activity up to 37 days. Therefore, ASP-enzymosomes are a promising
antileukemic drug. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Tecnologia Enzimática. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de Lisboa. Faculdade de Farmácia. Instituto de Investigação do Medicamento (iMed.ULisboa). LIsboa, Portugal. | pt_BR |
| Affilliation | Universidade de Lisboa. Faculdade de Farmácia. Instituto de Investigação do Medicamento (iMed.ULisboa). LIsboa, Portugal. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de Lisboa. Faculdade de Farmácia. Instituto de Investigação do Medicamento (iMed.ULisboa). LIsboa, Portugal. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Instituto de Tecnologia de Medicamentos. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Tecnologia Enzimática. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de Lisboa. Faculdade de Farmácia. Instituto de Investigação do Medicamento (iMed.ULisboa). LIsboa, Portugal. | pt_BR |
| Subject | Yeast asparaginase | pt_BR |
| Subject | ASP3 gene | pt_BR |
| Subject | ASP-enzymosomes | pt_BR |
| Subject | Nanoformulations | pt_BR |
| Subject | Enzymatic therapy | pt_BR |
| Subject | Leukemia treatment | pt_BR |
| e-ISSN | 1422-0067 | |
