| Author | Soares, Marco Aurélio | |
| Author | Araújo, Roberta Amália de Carvalho | |
| Author | Marini, Marjorie Mendes | |
| Author | Oliveira, Luciana Márcia de | |
| Author | Lima, Leonardo Gomes de | |
| Author | Alves, Viviane de Souza | |
| Author | Felipe, Maria Sueli Soares | |
| Author | Brigido, Marcelo Macedo | |
| Author | Soares, Celia Maria de Almeida | |
| Author | Silveira, Jose Franco da | |
| Author | Ruiz, Jeronimo Conceição | |
| Author | Cisalpino, Patrícia Silva | |
| Access date | 2022-02-01T13:27:36Z | |
| Available date | 2022-02-01T13:27:36Z | |
| Document date | 2015 | |
| Citation | SOARES, Marco Aurélio et al. dentification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, v. 16, n. 1, p. 376, 2015. doi: 10.1186/s12864-015-1564-7 | pt_BR |
| ISSN | 1471-2164 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50971 | |
| Sponsorship | FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO | pt_BR |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex | pt_BR |
| Type | Article | |
| DOI | 10.1186/s12864-015-1564-7 | |
| Abstract | Background: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes. Results: We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR. Conclusion: Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Programa de Pós-graduação em Bioinformática. Belo Horizonte, MG, Brazil/ Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Informática de Biossistemas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Biologia Geral. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade de Brasília. Laboratório de Biologia Molecular. Laboratório de Biologia Molecular. Brasília, DF, Brazil | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Informática de Biossistemas. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil /Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Programa de Pós-graduação em Bioinformática. Belo Horizonte, MG, Brazil | pt_BR |
