| Author | Paschoal, Ramon Gredilha | |
| Author | Viçosa, Alessandra Lifsitch | |
| Author | Suarez-Fontes, Ana Mácia | |
| Author | Vannier_Santos, Marcos André | |
| Author | Moreira, Davyson de Lima | |
| Access date | 2022-02-01T20:19:02Z | |
| Available date | 2022-02-01T20:19:02Z | |
| Document date | 2021 | |
| Citation | PASCHOAL, Ramon Gredilha et al. Desenvolvimento de Metodologia Analítica por Cromatografia em Fase Líquida de Alta Eficiência para Quantificação de Curcuminoides. Revista Virtual de Química, v. 13, n. 5, p. 1181-1188, 2021. | pt_BR |
| ISSN | 1984-6835 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50980 | |
| Language | por | pt_BR |
| Publisher | Sociedade Brasileira de Química | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Quantificação de Curcuminoides | pt_BR |
| Subject in Portuguese | Metodologia Analítica | pt_BR |
| Subject in Portuguese | Cromatografia | pt_BR |
| Subject in Portuguese | Desenvolvimento | pt_BR |
| Subject in Portuguese | Fase Líquida de Alta Eficiência | pt_BR |
| Title | Desenvolvimento de Metodologia Analítica por Cromatografia em Fase Líquida de Alta Eficiência para Quantificação de Curcuminoides | pt_BR |
| Alternative title | Development of Analytical Methodology by High-Performance Liquid Chromatography to Curcuminoids Quantification | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.21577/1984-6835.20210064 | |
| Abstract | Turmeric (Curcuma longa L.) is a species that has attracted great interest for containing bioactive
curcuminoids that have some pharmacological and physiological activities. Among them, antimalarial,
anti-inflammatory, antidepressant and anticancer effects that are mainly attributed to curcumin,
demethoxycurcumin and bisdemethoxycurcumin stand out. This work aimed the development of an
analytical methodology to optimization of the best conditions for sample preparation, chromatographic
separation, detection, identification and quantification of curcumin, demethoxycurcumin and
bisdemethoxycurcumin. The samples were weighed and solubilized to obtain a 200 μg/mL solution,
analyzed in triplicate, using the following chromatographic parameters: flow rate at 1.2 mL/min;
temperature at 50 oC; detection at 420 nm; total analysis time of 25 min and injection volume of 20 μL.
The developed method showed good selectivity, resolution, signal symmetry and capacity factor for
curcuminoids analysis. It is worth noting that the new method developed showed a scientific gain for
being more efficient and with the change of column, there was a gain in selectivity and resolution for the
separation of curcuminoids. In addition, the silica column modified with phenyl groups has a phase of
medium polarity and has greater selectivity in the separation of aromatic compounds. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Laboratório de Toxicologia Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Farmacotécnica Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Laboratório de Toxicologia Ambiental. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz, Farmanguinhos, Laboratório de Produtos Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Curcumin | pt_BR |
| Subject | Demethoxycurcumin | pt_BR |
| Subject | Bisdemethoxycurcumin | pt_BR |
| Subject | Analytical method | pt_BR |
| Subject | HPLC | pt_BR |
