| Author | Rodrigues, Flávia Guimarães | |
| Author | Santos, Maira Neves | |
| Author | Carvalho, Thiago Xavier Teixeira de | |
| Author | Rocha, Bruno Coelho | |
| Author | Riehle, Michael A | |
| Author | Pimenta, Paulo Filemon Paolucci | |
| Author | Abraham, E. G. | |
| Author | Jacobs-Lorena, M. | |
| Author | Brito, Cristiana Ferreira Alves de | |
| Author | Moreira, Luciano Andrade | |
| Access date | 2022-02-02T19:14:02Z | |
| Available date | 2022-02-02T19:14:02Z | |
| Document date | 2008 | |
| Citation | RODRIGUES, Flávia Guimarães et al. Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development. Insect Mol Biol., v. 17, n. 2, p. 175–183, 2008. Doi:10.1111/j.1365-2583.2008.00791.x. | pt_BR |
| ISSN | 0962-1075 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/50988 | |
| Sponsorship | CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO | pt_BR |
| Language | eng | pt_BR |
| Publisher | Blackwell Scientific For The Royal Entomological Society | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Expression of a mutated phospholipase A(2) in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1365-2583.2008.00791.x | |
| Abstract | The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A(2) (PLA(2)) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA(2) blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA(2) (mPLA(2)) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA(2 | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Department of Entomology. University of Arizona. Tucson, AZ, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Malaria Research Institute. Baltimore, MD, USA | pt_BR |
| Affilliation | Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Malaria Research Institute. Baltimore, MD, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | transformation | pt_BR |
| Subject | bee venom phospholipase A(2) | pt_BR |
| Subject | malaria | pt_BR |
| Subject | piggyBac | pt_BR |
| Subject | fitness | pt_BR |
