| Author | Campelo, Thales Alves | |
| Author | Sousa, Paulo Rafael Cardoso de | |
| Author | Nogueira, Lucas de Lima | |
| Author | Frota, Cristiane Cunha | |
| Author | Antas, Paulo Renato Zuquim | |
| Access date | 2022-02-07T20:00:11Z | |
| Available date | 2022-02-07T20:00:11Z | |
| Document date | 2021 | |
| Citation | CAMPELO, Thales Alves et al. Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?. Access Microbiology, v. 3, 000245, p. 1 - 15, Aug. 2021. | pt_BR |
| ISSN | 2516-8290 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51064 | |
| Language | eng | pt_BR |
| Publisher | Microbiology Society | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | Tuberculose resistente a medicamentos | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Técnicas de amplificação de ácido nucleico | pt_BR |
| Title | Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far? | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1099/acmi.0.000245 | |
| Abstract | Tuberculosis (TB) affects around 10 million people worldwide in 2019. Approximately 3.4 % of new TB cases are multidrug-resistant.
The gold standard method for detecting Mycobacterium tuberculosis, which is the aetiological agent of TB, is still
based on microbiological culture procedures, followed by species identification and drug sensitivity testing. Sputum is the most
commonly obtained clinical specimen from patients with pulmonary TB. Although smear microscopy is a low-cost
and widely
used method, its sensitivity is 50–60 %. Thus, owing to the need to improve the performance of current microbiological tests
to provide prompt treatment, different methods with varied sensitivity and specificity for TB diagnosis have been developed.
Here we discuss the existing methods developed over the past 20 years, including their strengths and weaknesses. In-house
and commercial methods have been shown to be promising to achieve rapid diagnosis. Combining methods for mycobacterial
detection systems demonstrates a correlation of 100 %. Other assays are useful for the simultaneous detection of M. tuberculosis
species and drug-related
mutations. Novel approaches have also been employed to rapidly identify and quantify total
mycobacteria RNA, including assessments of global gene expression measured in whole blood to identify the risk of TB. Spoligotyping,
mass spectrometry and next-generation
sequencing are also promising technologies; however, their cost needs to be
reduced so that low- and middle-income
countries can access them. Because of the large impact of M. tuberculosis infection on
public health, the development of new methods in the context of well-designed
and -controlled clinical trials might contribute
to the improvement of TB infection control. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Ceará. Faculade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Drug-resistant tuberculosis | pt_BR |
| Subject | Nucleic acid amplification techniques | pt_BR |
| Subject | Tuberculosis | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
