| Author | Morgado, Sergio | |
| Author | Fonseca, Érica | |
| Author | Vicente, Ana Carolina | |
| Access date | 2022-02-08T14:36:14Z | |
| Available date | 2022-02-08T14:36:14Z | |
| Document date | 2021 | |
| Citation | MORGADO, Sergio; FONSECA, Èrica; VICENTE, Ana Carolina. Genomic epidemiology of rifampicin ADP‑ribosyltransferase (Arr) in the Bacteria domain. Scientific Reports, v. 11, 19775, p. 1 - 9, 2021. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51074 | |
| Language | eng | pt_BR |
| Publisher | Nature Portfolio | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Epidemiologia genômica | pt_BR |
| Subject in Portuguese | Rifampicina | pt_BR |
| Subject in Portuguese | ADP-ribosiltransferase (Arr) | pt_BR |
| Subject in Portuguese | domínio Bactérias | pt_BR |
| Title | Genomic epidemiology of rifampicin ADP‑ribosyltransferase (Arr) in the Bacteria domain | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-021-99255-3 | |
| Abstract | Arr is an ADP-ribosyltransferase enzyme primarily reported in association with rifamycin resistance,
which has been used to treat tuberculosis in addition to Gram-positive infections and, recently,
pan-resistant Gram-negative bacteria. The arr gene was initially identified on the Mycolicibacterium
smegmatis chromosome and later on Proteobacteria plasmids. This scenario raised concerns on the
distribution and spread of arr, considering the Bacteria domain. Based on 198,082 bacterial genomes/
metagenomes, we performed in silico analysis, including phylogenetic reconstruction of Arr in
different genomic contexts. Besides, new arr alleles were evaluated by in vitro analysis to assess
their association with rifampin resistance phenotype. The arr gene was prevalent in thousands of
chromosomes and in hundreds of plasmids from environmental and clinical bacteria, mainly from
the phyla Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidetes. Furthermore, this gene
was identified in other and new genomic contexts. Interestingly, Arr sequences associated with
rifampin resistance were distributed across all phylogeny, indicating that, despite the diversity,
their association with rifampin resistance phenotype were maintained. In fact, we found that the
key residues were highly conserved. In addition, other analyzes have raised evidence of another Arr
function, which is related to guanidine metabolism. Finally, this scenario as a whole also suggested
the Actinobacteria phylum as a potential ancestral source of arr within the Bacteria domain. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Genomic epidemiology | pt_BR |
| Subject | Rifampicin | pt_BR |
| Subject | ADP‑ribosyltransferase (Arr) | pt_BR |
| Subject | Bacteria domain | pt_BR |
