| Author | Oliveira, Amanda Gleyce Lima de | |
| Author | Sudré, Adriana Pittella | |
| Author | Bomfim, Teresa Cristina Bergamo do | |
| Author | Santos, Helena Lúcia Carneiro | |
| Access date | 2022-02-09T14:59:07Z | |
| Available date | 2022-02-09T14:59:07Z | |
| Document date | 2021 | |
| Citation | OLIVEIRA, Amanda Gleyce Lima de et al. Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype. Plos One, v. 16, n. 8, 0255087, p. 1 - 13, Aug. 2021. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51108 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Caracterização molecular | pt_BR |
| Subject in Portuguese | Cryptosporidium spp. | pt_BR |
| Subject in Portuguese | Cães e gatos | pt_BR |
| Subject in Portuguese | Rio de Janeiro | pt_BR |
| Subject in Portuguese | Revela potencial zoonótico | pt_BR |
| Subject in Portuguese | Genótipo | pt_BR |
| Title | Molecular characterization of Cryptosporidium spp. in dogs and cats in the city of Rio de Janeiro, Brazil, reveals potentially zoonotic species and genotype | pt_BR |
| Type | Article | |
| DOI | 10.1371/ journal.pone.0255087 | |
| Abstract | Intestinal cryptosporidiosis is a diarrheal disease caused by protists of genus Cryptosporidium
that infect a wide variety of hosts, primarily vertebrates. Due to the close contact
between humans and their companion animals, especially dogs and cats, there is concern
about the potential for zoonotic transmission of this enteric protozoan parasite by infected
animals. This study aimed to perform a microscopic and molecular diagnosis of Cryptosporidium
spp. in fecal samples from domiciled dogs and cats. One hundred and nineteen fecal
samples were processed using sugar centrifugal flotation followed by molecular detection of
Cryptosporidium spp. DNA using nested PCR. Subtyping of isolates positive for C. parvum
was performed by sequence analysis of the 60 kDa glycoprotein gene (GP60). Cryptosporidium
oocysts were detected in 7.8% (5/64) and 5.4% (3/55) of the fecal samples from dogs
and cats, respectively. Cryptosporidium canis (n = 3) and C. parvum (n = 2) were the main
species found in dogs, whereas C. felis (n = 3) was prevalent in cats. Subtype IIaA17G2R2
(potentially zoonotic) was identified in samples positive for C. parvum. Despite the low prevalence
of Cryptosporidium observed in the domiciled dogs and cats, the presence of potentially
zoonotic C. parvum in dogs evidences a public health concern. Further research is
needed to better understand the epidemiology, source, and potential impacts of Cryptosporidium
infection in cats and dogs. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Parasitologia. Seropédica, Rio de Janeiro, Brasil. l | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Rural do Rio de Janeiro. Departamento de Parasitologia. Instituto de Veterinária. Seropédica, Rio de Janeiro, Brasil. l | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Molecular characterization | pt_BR |
| Subject | Cryptosporidium spp. | pt_BR |
| Subject | Dogs | pt_BR |
| Subject | Cats | pt_BR |
| Subject | Potentially zoonotic species | pt_BR |
| Subject | Genotype | pt_BR |
| Subject | Rio de Janeiro | pt_BR |
| Subject | Brasil | pt_BR |
