| Author | Gomes, Juliana Moreira Mendonça | |
| Author | Charlie-Silva, Ives | |
| Author | Santos, Anderson Kenedy | |
| Author | Resende, Rodrigo Ribeiro | |
| Author | Estanislau, Juliana de Assis Silva Gomes | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Corrêa Junior, José Dias | |
| Access date | 2022-02-15T17:55:07Z | |
| Available date | 2022-02-15T17:55:07Z | |
| Document date | 2021 | |
| Citation | GOMES, Juliana Moreira Mendonça et al. Flow cytometry in the analysis of hematological parameters of tilapias: applications in environmental aquatic toxicology. Environ Sci Pollut Res Int., v. 28, n. 5, p. 6242-6248, 2021.doi: 10.1007/s11356-020-12119-7 | pt_BR |
| ISSN | 0944-1344 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51222 | |
| Language | eng | pt_BR |
| Publisher | Springer International | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Flow cytometry in the analysis of hematological parameters of tilapias: applications in environmental aquatic toxicology | pt_BR |
| Type | Article | |
| Abstract | Blood tissue has been used to assess animal health and the environment in which they live. This tissue is easily acquired and has the ability to respond to various adverse conditions. Several techniques have been employed in the detection of xenobiotic-induced cell damage in blood cells. In general, traditionally used technologies, such as cellular analysis in blood smears, are time-consuming and require great analytical capacity. The present study proposes flow cytometry as a method to detect changes in blood cell populations. Tilapia (Oreochromis niloticus) was selected as a model for plotting the profile of fish blood cell populations after exposure to xenobiotics without euthanizing animals or using cell markers. Populations of erythrocytes and lymphocytes were detected only by combining the techniques of FACSAria cell sorting and light microscopy. Systemic deleterious effects were found through blood analysis, such as an increased lymphocyte-rich population at 48 h of exposure followed by a subsequent decrease. Moreover, the time-dependent expression of Nrf2 suggests its participation in increased membrane disruption, indicating it has a central role in erythrocyte lifespan. The present results shed light on the viability of using flow cytometry for blood analysis of living fish. | pt_BR |
| Affilliation | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Department of Pharmacology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Departamento de Biochemistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Departamento de Biochemistry. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil. | pt_BR |
| Subject | Blood | pt_BR |
| Subject | Cell sorting | pt_BR |
| Subject | Erythrocyte | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Subject | Lymphocyte | pt_BR |
| Subject | Nrf2 | pt_BR |
| Subject | Tilapia | pt_BR |
