Author | Queiroz, Edson Alexandre de | |
Author | Medeiros, Nayara Ingrid | |
Author | Mattos, Rafael Teixeira de | |
Author | Pinto, Bruna Fernandes | |
Author | Carvalho, Ana Paula Mendes | |
Author | Dutra, Walderez Ornelas | |
Author | Lana, Francisco Carlos Félix | |
Author | Oliveira, Rodrigo Corrêa de | |
Author | Estanislau, Juliana de Assis Silva Gomes | |
Access date | 2022-02-17T16:32:14Z | |
Available date | 2022-02-17T16:32:14Z | |
Document date | 2021 | |
Citation | QUEIROZ, Edson Alexandre de et al. CCL2 and IFN-γ serum levels as biomarkers for subclinical infection in household contacts of leprosy patients. Microb Pathog., v. 150, 104725, 2021. doi: 10.1016/j.micpath.2020.104725. | pt_BR |
ISSN | 0882-4010 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/51272 | |
Language | eng | pt_BR |
Publisher | Academic Press | pt_BR |
Rights | restricted access | pt_BR |
Title | CCL2 and IFN-γ serum levels as biomarkers for subclinical infection in household contacts of leprosy patients | pt_BR |
Type | Article | pt_BR |
Abstract | Leprosy, also known as Hansen\'s disease, is a long-term infection by the bacteria Mycobacterium leprae, and actually still persists as a serious public health problem. The clinical parameters are used for diagnosis, however, some studies have indicated the selection of a set of biomarkers of subclinical infection, both serological and cellular, that allow the early diagnosis. Some cytokines and chemokines have been differentially expressed in index cases (paucibacillary and multibacillary patients) and household contacts (HHC), and may present a potential biomarker of M. leprae subclinical infection. Thus, the aim of this study was to analyze the variations in the profile of cytokines and chemokines, longitudinally, between index cases and their household contacts with a view to identifying possible biomarkers with differential expression, which may guide the early subclinical infection in household contacts. A longitudinal study was carried out between 2014 and 2015. The serum levels of the cytokines and chemokines were measured in all patient samples by CBA (Cytometric Bead Array). We observed a reduction of IL-4 and IL-17 expression of HHC group in the second evaluation (T1), as also a reduction of IL-17 in MB. We observed increased expression of IL-2 in PB patients as well. HHC, PB and MB showed a similar reduction profile of the chemokines CXCL8, CXCL9 and CXCL10 from T0 to T1. Interestingly, only serological levels of CCL2 are increased after a follow-up of HHC group, and this group, but not PB and MB patients, showed a significant association and a negative correlation between CCL2 and IFN-gamma. The present study showed for the first time a similarity in the immunological scenario between HHC, PB and MB patients. In addition, this work highlights CCL2 chemokine in association with IFN-gamma as possible biomarkers of subclinical infection of HHC, as also a parameter of early infection monitoring. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Escola de Enfermagem. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil. | pt_BR |
Subject | Leprosy | pt_BR |
Subject | Household contact | pt_BR |
Subject | Subclinical infection | pt_BR |
Subject | Immunological biomarkers | pt_BR |
Subject | CCL2 | pt_BR |
Subject | IFN-γ | pt_BR |