| Author | Gonçales, Relber Aguiar | |
| Author | Salamanca, Ayda Lm | |
| Author | Rb Júnior, Luiz | |
| Author | Silva, Kleber Sf E | |
| Author | Vasconcelos, Elton Jr de | |
| Author | Reis, Thaila F Dos | |
| Author | Castro, Ricardo C | |
| Author | Ruy, Patrícia de C | |
| Author | Romagnoli, Bárbara | |
| Author | Ruiz, Jerônimo Conceição | |
| Author | Pereira, Maristela | |
| Author | Soares, Célia M de A | |
| Author | Coelho, Paulo Sr | |
| Access date | 2022-02-21T12:19:25Z | |
| Available date | 2022-02-21T12:19:25Z | |
| Document date | 2021 | |
| Citation | GONÇALES, Relber Aguiar et al. In silico identification of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides spp. Future Microbiology, v. 16, n. 8, p. 589-606, 2021. do:10.2217/fmb-2020-0282 | pt_BR |
| ISSN | 1746-0913 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51321 | |
| Language | eng | pt_BR |
| Publisher | Future Medicine | pt_BR |
| Rights | restricted access | pt_BR |
| Title | In silico identification of glycosylphosphatidylinositol-anchored proteins in Paracoccidioides spp | pt_BR |
| Type | Article | |
| DOI | 10.2217/fmb-2020-0282 | |
| Abstract | Aim: To predict glycosylphosphatidylinositol (GPI)-anchored proteins in the genome of Paracoccidioides brasiliensis and Paracoccidioides lutzii. Materials & methods: Five different bioinformatics tools were used for predicting GPI-anchored proteins; we considered as GPI-anchored proteins those detected by at least two in silico analysis methods. We also performed the proteomic analysis of P. brasiliensis cell wall by mass spectrometry. Results: Hundred GPI-anchored proteins were predicted in P. brasiliensis and P. lutzii genomes. A series of 57 proteins were classified in functional categories and 43 conserved proteins were reported with unknown functions. Four proteins identified by in silico analyses were also identified in the cell wall proteome. Conclusion: The data obtained in this study are important resources for future research of GPI-anchored proteins in Paracoccidioides spp. to identify targets for new diagnostic tools, drugs and immunological tests. | pt_BR |
| Affilliation | Life and Health Sciences Research Institute. School of Medicine. University of Minho. Braga, Portugal/ ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas II. Departamento de Bioquímica e Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Department of Cellular & Molecular Biology and Pathogenic Bioagents. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas II. Departamento de Bioquímica e Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | College of Veterinary Medicine. Western University of Health Sciences. Pomona, CA , USA | pt_BR |
| Affilliation | Universidade de São Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Department of Cellular & Molecular Biology and Pathogenic Bioagents. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Department of Cellular & Molecular Biology and Pathogenic Bioagents. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas II. Departamento de Bioquímica e Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas II. Departamento de Bioquímica e Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Department of Cellular & Molecular Biology and Pathogenic Bioagents. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Subject | cell wall | pt_BR |
| Subject | glycosylphosphatidylinositol-anchored proteins | pt_BR |
| Subject | Paracoccidioides brasiliensis | pt_BR |
| Subject | Paracoccidioides lutzii | pt_BR |
| Subject | paracoccidioidomycosis | pt_BR |
