Author | Salas, Nehuén | |
Author | Coceres, Veronica M. | |
Author | Melo, Tuanne dos Santos | |
Author | Pereira‑Neves, Antonio | |
Author | Maguire, Vanina G. | |
Author | Rodriguez, Tania M. | |
Author | Sabatke, Bruna | |
Author | Ramirez, Marcel Ivan | |
Author | Sha, Jihui | |
Author | Wohlschlegel, James A. | |
Author | Miguel, Natalia de | |
Access date | 2022-02-25T16:06:16Z | |
Available date | 2022-02-25T16:06:16Z | |
Document date | 2022 | |
Citation | SALAS, Nehuén et al. VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles. Cellular and Molecular Life Sciences, v. 79, p. 1-16, 2022. | pt_BR |
ISSN | 1420-9071 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/51496 | |
Language | por | pt_BR |
Publisher | Springer | pt_BR |
Rights | open access | pt_BR |
Title | VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s00018-021-04083-3 | |
Abstract | Trichomonas vaginalis is a common sexually transmitted extracellular parasite that adheres to epithelial cells in the human urogenital tract. Extracellular vesicles (EVs) have been described as important players in the pathogenesis of this parasite as they deliver proteins and RNA into host cells and modulate parasite adherence. EVs are heterogeneous membrane vesicles released from virtually all cell types that collectively represent a new dimension of intercellular communication. The Endosomal Sorting Complex Required for Transport (ESCRT) machinery contributes to several key mechanisms in which it reshapes membranes. Based on this, some components of the ESCRT have been implicated in EVs biogenesis in other cells. Here, we demonstrated that VPS32, a member of ESCRTIII complex, contribute to the biogenesis and cargo sorting
of extracellular vesicles in the parasite T. vaginalis. Moreover, we observe that parasites overexpressing VPS32 have a striking increase in adherence to host cells compared to control parasites; demonstrating a key role for this protein in mediating host: parasite interactions. These results provide valuable information on the molecular mechanisms involved in extracellular vesicles biogenesis, cargo-sorting, and parasite pathogenesis. | pt_BR |
Affilliation | Instituto Tecnológico de Chascomús. Laboratorio de Parásitos Anaerobios. Chascomús, Buenos Aires, Argentina. | pt_BR |
Affilliation | Instituto Tecnológico de Chascomús. Laboratorio de Parásitos Anaerobios. Chascomús, Buenos Aires, Argentina. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil. | pt_BR |
Affilliation | Instituto Tecnológico de Chascomús. Laboratorio de Parásitos Anaerobios. Chascomús, Buenos Aires, Argentina. | pt_BR |
Affilliation | Instituto Tecnológico de Chascomús. Laboratorio de Parásitos Anaerobios. Chascomús, Buenos Aires, Argentina. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Department of Biological Chemistry. University of California. Los Angeles, USA. | pt_BR |
Affilliation | Department of Biological Chemistry. University of California. Los Angeles, USA. | pt_BR |
Affilliation | Instituto Tecnológico de Chascomús. Laboratorio de Parásitos Anaerobios. Chascomús, Buenos Aires, Argentina. | pt_BR |
Subject | Extracellular Vesicles | pt_BR |
Subject | ESCRTIII complex | pt_BR |
Subject | Endosomal Sorting Complexes Required for Transport | pt_BR |
Subject | Host-Parasite Interactions | pt_BR |
Subject | VPS32 | pt_BR |
Subject in Spanish | Vesículas Extracelulares | pt_BR |
Subject in Spanish | Complejos de Clasificación Endosomal Requeridos para el Transporte | pt_BR |
Subject in Spanish | Interacciones Huésped-Parásitos | pt_BR |
Subject in Spanish | VPS32 | pt_BR |
Subject in French | Vésicules extracellulaires | pt_BR |
Subject in French | Complexes de tri endosomique requis pour le transport | pt_BR |
Subject in French | Interactions hôte-parasite | pt_BR |
Subject in French | VPS32 | pt_BR |
DeCS | Vesículas Extracelulares | pt_BR |
DeCS | Complexos Endossomais de Distribuição Requeridos para Transporte | pt_BR |
DeCS | Interações Hospedeiro-Parasita | pt_BR |
DeCS | Trichomonas vaginalis | pt_BR |