| Author | Alves, Felipe de Andrade Vieira | |
| Author | Oliveira, Lucca de Lima S. | |
| Author | Salomão, Natáia Gedeão | |
| Author | Provance Jr., David William | |
| Author | Oliveira, Carlos Alberto Basilio de | |
| Author | Oliveira, Rodrigo Basílio de | |
| Author | Moragas, Leandro Junqueira | |
| Author | Carvalho, Jorge José de | |
| Author | Borges, Ronaldo Mohana | |
| Author | Rabello, Kíssila | |
| Author | Paes, Marciano Viana | |
| Access date | 2022-03-05T17:59:50Z | |
| Available date | 2022-03-05T17:59:50Z | |
| Document date | 2022 | |
| Citation | ALVES, Felipe de Andrade Vieira et al. Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis. Plos One, v. 17, n. 1, e0262785, p. 1 - 16, Jan. 2022. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51567 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Citocinas | pt_BR |
| Subject in Portuguese | Mediadores inflamatórios | pt_BR |
| Subject in Portuguese | Marcadores | pt_BR |
| Subject in Portuguese | Danos intersticiais | pt_BR |
| Subject in Portuguese | Pâncreas | pt_BR |
| Subject in Portuguese | Febre da Dengue | pt_BR |
| Subject in Portuguese | Pancreatite aguda | pt_BR |
| Title | Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0262785 | |
| Abstract | Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage.
This study examined the pancreas from two fatal cases of DENV for histopathological
changes as well as for the detection of cytokines, and other inflammatory mediators. Tissue
sections were prepared for examination by ultrastructural and histopathological techniques.
Sections from the pancreas of non-infected individuals were prepared in parallel as a control.
The presence of viral replication in macrophages was detected by co-staining for the
proteins NS3 and CD68 by immunofluorescence. Immunohistochemistry was used to detect
cells that expressed cytokines and inflammatory mediators to characterize the inflammatory
response. Edema, acinar necrosis and fibrosis areas associated with a mononuclear infiltrate
were found in infected tissues. The major site of virus replication appeared to be macrophages
based on their exclusive presentation of the viral protein NS3. Pancreatic tissues
from the infected individuals also displayed increased levels of high mobility group box-1,
caspase-3, gelatinase B and tumor necrosis factor alpha compared to controls. The presence
of virus replicating macrophages in the pancreas was associated with multiple
changes in tissue structure that included elevated levels of cytokines and inflammatory
markers that may differentiate acute pancreatitis due to DENV infections from other causes. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Anatomia Patológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Anatomia Patológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Laboratório de Ultraestrutura e Biologia Tecidual. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Laboratório de Genômica Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Laboratório de Ultraestrutura e Biologia Tecidual. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Inflammatory mediators | pt_BR |
| Subject | Markers | pt_BR |
| Subject | Interstitial damage | pt_BR |
| Subject | Pancreas | pt_BR |
| Subject | Dengue fever | pt_BR |
| Subject | acute pancreatitis | pt_BR |
