| Author | Freitas, Maria Audilene de | |
| Author | Cruz, Rafael Pereira da | |
| Author | Santos, Antonia Thassya Lucas dos | |
| Author | Bezerra, José Weverton Almeida | |
| Author | Machado, Antonio Júdson Targino | |
| Author | Santos, Joycy Francely Sampaio dos | |
| Author | Rocha, Janaina Esmeraldo | |
| Author | Boligon, Aline Augusti | |
| Author | Bezerra, Camila Fonseca | |
| Author | Freitas, Thiago Sampaio de | |
| Author | Silva, Maria Karollyna do Nascimento | |
| Author | Mendonça, Ana Cleide Alcântara Morais | |
| Author | Costa, José Galberto Martins da | |
| Author | Coutinho, Henrique Douglas Melo | |
| Author | Cunha, Francisco Assis Bezerra da | |
| Author | Ribeiro Filho, Jaime | |
| Author | Braga, Maria Flaviana Bezerra Morais | |
| Access date | 2022-03-11T13:40:54Z | |
| Available date | 2022-03-11T13:40:54Z | |
| Document date | 2022 | |
| Citation | Freitas, Maria Audilene de et al. HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae). 3 Biotech, v. 12, n. 1, p. 1-16, 2022. | pt_BR |
| ISSN | 2190-5738 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51683 | |
| Description | Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Federal de Santa Maria. Santa Maria, RS, Brasil. Universidade Federal de Pernambuco. Recife, PE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. Universidade Regional do Cariri. Crato, CE, Brasil. | pt_BR |
| Sponsorship | Fundação Cearense de Apoio ao Desenvolvimento (FUNCAP). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Financiadora de Estudos e Projetos (FINEP). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Springer | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Compostos fitoquímicos | pt_BR |
| Subject in Portuguese | Antibacterianos | pt_BR |
| Subject in Portuguese | Antifúngicos | pt_BR |
| Title | HPLC–DAD analysis and antimicrobial activities of Spondias mombin L. (Anacardiaceae) | pt_BR |
| Type | Article | pt_BR |
| DOI | doi.org/10.1007/s13205-022-03126-1 | |
| Abstract | Spondias mombin is used in the folk medicine for the treatment of diarrhea and dysentery, indicating that extracts obtained from this species may present pharmacological activities against pathogenic microorganisms. The purpose of this work was to investigate the chemical composition and evaluate the antimicrobial activity of extracts obtained from the leaves (aqueous) and bark (hydroethanolic) of S. mombin both as single treatments and in combination with conventional drugs. Following a qualitative chemical prospection, the extracts were analyzed by HPLC–DAD. The antimicrobial activities were evaluated by microdilution. The combined activity of drugs and extracts was verified by adding a subinhibitory concentration of the extract in the presence of variable drug concentrations. The Minimum Fungicidal Concentration (MFC) was determined by a subculture of the microdilution test, while the effect of the in vitro treatments on morphological transition was analyzed by subculture in moist chambers. While the qualitative analysis detected the presence of phenols and flavonoids, the HPLC analysis identified quercetin, caffeic acid, and catechin as major components in the leaf extract, whereas kaempferol and quercetin were found as major compounds in the bark extract. The extracts showed effective antibacterial activities only against the Gram-negative strains. With regard to the combined activity, the leaf extract potentiated the action of gentamicin and imipenem (against Staphylococcus aureus), while the bark extract potentiated the effect of norfloxacin (against S. aureus), imipenem (against Escherichia coli), and norfloxacin (against Pseudomonas aeruginosa). A more significant antifungal (fungistatic) effect was achieved with the bark extract (even though at high concentrations), which further enhanced the activity of fluconazole. The extracts also inhibited the emission of filaments by Candida albicans and Candida tropicalis. Together, these findings suggest that that the extract constituents may act by favoring the permeability of microbial cells to conventional drugs, as well as by affecting virulence mechanisms in Candida strains. | pt_BR |
| Affilliation | "Múltipla ver em Notas" | pt_BR |
| Subject | Phytochemicals | pt_BR |
| Subject | Anti-Bacterial agents | pt_BR |
| Subject | Antifungal agents | pt_BR |
| Subject in Spanish | Fitoquímicos | pt_BR |
| Subject in Spanish | Antibacterianos | pt_BR |
| Subject in Spanish | Antifúngicos | pt_BR |
| Subject in French | Composés phytochimiques | pt_BR |
| Subject in French | Antibactériens | pt_BR |
| Subject in French | Antifongiques | pt_BR |
