| Author | Alvarez, Luis Carlos Salazar | |
| Author | Lizcano, Omaira Vera | |
| Author | Barros, Dayanne Kamylla Alves da Silva | |
| Author | Silva, Djane Clarys Baia da | |
| Author | Monteiro, Wuelton Marcelo | |
| Author | Pimenta, Paulo Filemon Paolluci | |
| Author | Lacerda, Marcus Vinicius Guimarães de | |
| Author | Costa, Fabio Trindade Maranhão | |
| Author | Lopes, Stefanie Costa Pinto | |
| Access date | 2022-03-21T15:40:16Z | |
| Available date | 2022-03-21T15:40:16Z | |
| Document date | 2021 | |
| Citation | ALVAREZ, Luis Carlos Salazar et al. Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells. Front Cell Infect Microbiol., v. 11, 614985, 2021. doi: 10.3389/fcimb.2021.614985. | pt_BR |
| ISSN | 2235-2988 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51771 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media AS | pt_BR |
| Rights | open access | pt_BR |
| Title | Plasmodium vivax Gametocytes Adherence to Bone Marrow Endothelial Cells | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3389/fcimb.2021.614985 | |
| Abstract | In a Plasmodium vivax infection, it was shown a proportionally increased on gametocyte distribution within the bone marrow aspirant, suggesting a role of this organ as a reservoir for this parasite stage. Here, we evaluated the ex vivo cytoadhesive capacity of P. vivax gametocytes to bone marrow endothelial cells (HBMEC) and investigated the involvement of some receptors in the cytoadhesion process by using transfected CHO cells (CHO-ICAM1, CHO-CD36 and CHO-VCAM), wild type (CHO-K1) or deficient in heparan and chondroitin sulfate (CHO-745). Ex-vivo cytoadhesion assays were performed using a total of 44 P. vivax isolates enriched in gametocyte stages by Percoll gradient in the different cell lines. The majority of isolates (88.9%) were able to adhere to HBMEC monolayer. ICAM1 seemed to be the sole receptor significantly involved. CD-36 was the receptor with higher adhesion rate, despite no significance was noticed when compared to CHO-745. We demonstrated that gametocyte P. vivax adheres ex vivo to bone marrow endothelial cells. Moreover, P. vivax gametocytes display the ability to adhere to all CHO cells investigated, especially to CHO-ICAM1. These findings bring insights to the comprehension of the role of the bone marrow as a P. vivax reservoir and the potential impact on parasite transmission to the vector. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brazil/Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/ Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil/ Grupo de Investigación QUIBIO. Facultad de Ciencias Básicas. Universidad Santiago de Cali. Cali, Colombia. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leonidas & Maria Deane. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Programa de Pós-Graduação em Medicina Tropical. Manaus, AM, Brazil | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leonidas & Maria Deane. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Universidade de Campinas. Departamento de Genética, Evolução, Microbiologia e Imunologia. Laboratório de Doenças Tropicais Prof. Dr. Luiz Jacintho da Silva. Campinas, SP, Brazil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto Leonidas & Maria Deane. Manaus, AM, Brazil. | pt_BR |
| Subject | Malaria | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
| Subject | bone marrow | pt_BR |
| Subject | cytoadhesion; gametocytes. | pt_BR |
