| Author | Oliveira, Simone S. C. | |
| Author | Elias, Camila G. R. | |
| Author | Dias, Felipe A. | |
| Author | Lopes, Angela H. | |
| Author | d`Avila-Levy, Claudia M. | |
| Author | Santos, Andre´ L. S. | |
| Author | Branquinha, Marta H. | |
| Access date | 2022-03-27T12:23:39Z | |
| Available date | 2022-03-27T12:23:39Z | |
| Document date | 2022 | |
| Citation | OLIVEIRA, Simone S. C. et al. The Enhanced Expression of Cruzipain-Like Molecules in the Phytoflagellate Phytomonas serpens Recovered From the Invertebrate and Plant Hosts. Frontiers in Cellular and Infection Microbiology, v. 11, Article 819133, p. 1 - 10, Jan. 2002. | pt_BR |
| ISSN | 2235-2988 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/51894 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Phytomonas serpens | pt_BR |
| Subject in Portuguese | Oncopeltus fasciatus | pt_BR |
| Subject in Portuguese | Tomate | pt_BR |
| Subject in Portuguese | Interação | pt_BR |
| Subject in Portuguese | Vetor invertebrado | pt_BR |
| Subject in Portuguese | Cruzipain | pt_BR |
| Title | The Enhanced Expression of Cruzipain-Like Molecules in the Phytoflagellate Phytomonas serpens Recovered From the Invertebrate and Plant Hosts | pt_BR |
| Type | Article | |
| DOI | 10.3389/fcimb.2021.819133 | |
| Abstract | Phytomonas serpens is a protozoan parasite that alternates its life cycle between two
hosts: an invertebrate vector and the tomato fruit. This phytoflagellate is able to synthesize
proteins displaying similarity to the cysteine peptidase named cruzipain, an important
virulence factor from Trypanosoma cruzi, the etiologic agent of Chagas disease. Herein,
the growth of P. serpens in complex medium (BHI) supplemented with natural tomato
extract (NTE) resulted in the increased expression of cysteine peptidases, as verified by
the hydrolysis of the fluorogenic substrate Z-Phe-Arg-AMC and by gelatin-SDS-PAGE.
Phytoflagellates showed no changes in morphology, morphometry and viability, but the
proliferation was slightly reduced when cultivated in the presence of NTE. The enhanced
proteolytic activity was accompanied by a significant increase in the expression of
cruzipain-like molecules, as verified by flow cytometry using anti-cruzipain antibodies. In
parallel, parasites incubated under chemically defined conditions (PBS supplemented with
glucose) and added of different concentration of NTE revealed an augmentation in the
production of cruzipain-like molecules in a typically dose-dependent way. Similarly, P.
serpens recovered from the infection of mature tomatoes showed an increase in the
expression of molecules homologous to cruzipain; however, cells showed a smaller size
compared to parasites grown in BHI medium. Furthermore, phytoflagellates incubated
with dissected salivary glands from Oncopeltus fasciatus or recovered from the
hemolymph of infected insects also showed a strong enhance in the expression of
cruzipain-like molecules that is more relevant in the hemolymph. Collectively, our results showed that cysteine peptidases displaying similarities to cruzipain are more expressed
during the life cycle of the phytoflagellate P. serpens both in the invertebrate and
plant hosts. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Bioquíımica de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Bioquíımica de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química Programa de Pós-Grauação em Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Phytomonas serpens | pt_BR |
| Subject | Tomato | pt_BR |
| Subject | Oncopeltus fasciatus | pt_BR |
| Subject | Interaction | pt_BR |
| Subject | Invertebrate vector | pt_BR |
| Subject | Cruzipain | pt_BR |
