| Author | Santos, Laís de Macêdo Ferreira | |
| Author | Barreto, Breno Cardim | |
| Author | Quadros, Helenita Costa | |
| Author | Meira, Cássio Santana | |
| Author | Carvalho, Rafaela de Siqueira Ferraz | |
| Author | Rebouças, Juliana de Souza | |
| Author | Macambira, Simone Garcia | |
| Author | Vasconcelos, Juliana Fraga | |
| Author | Souza, Bruno Solano de Freitas | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Magalhães, Nereide Stela Santos | |
| Author | Formiga, Fabio Rocha | |
| Access date | 2022-04-08T13:44:54Z | |
| Available date | 2022-04-08T13:44:54Z | |
| Document date | 2022 | |
| Citation | SANTOS, Laís de Macêdo et al. Tissue response and retention of micro- and nanosized liposomes in infarcted mice myocardium after ultrasound-guided transthoracic injection. European Journal of Pharmaceutics and Biopharmaceutics, v. 173, p. 1-9, 2022. | pt_BR |
| ISSN | 0939-6411 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/52127 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Infarto do Miocárdio | pt_BR |
| Subject in Portuguese | Lipossomos | pt_BR |
| Subject in Portuguese | Doença do Sistema de Condução Cardíaco | pt_BR |
| Title | Tissue response and retention of micro- and nanosized liposomes in infarcted mice myocardium after ultrasound-guided transthoracic injection | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.ejpb.2022.03.005 | |
| Abstract | Different carrier systems have been investigated for myocardial delivery of biopharmaceuticals for heart disease. Here, we aimed to evaluate the heart retention and tissue response of liposomes intended for cardiac drug delivery. Liposomes were produced by the lipid thin film hydration method followed by sonication. Cytocompatibility tests were performed in murine L929 fibroblasts and H2c9 cardiomyocytes using the Alamar Blue assay. In vivo experiments were assessed in a model of myocardial infarction induced by isoproterenol in mice. Cardiac delivery of fluorescent liposomes (rhodamine B-labeled) with different mean sizes (165 nm, 468 nm, 1551 nm and 1954 nm) was performed by ultrasound-guided transthoracic injection. After three days, mice were euthanized for histological evaluation using optical and fluorescence microscopy. No cytotoxic lipid concentrations were determined in the range 9.3 – 120 µM for fibroblasts and cardiomyocytes exposed to liposomes. In vivo, large liposomes induced significant inflammation in myocardium compared with the control group (p < 0.0001). In contrast, heart mice injected with 468 nm-sized liposomes exhibited a lower number of inflammatory cells. Still, a greater tissue retention 72 h post-injection was found. Therefore, this study demonstrated the feasibility of the echocardiography-guided percutaneous injection to deliver liposomes successfully into the myocardium in a minimally invasive manner. In addition, these findings indicate the potential of liposomes as carriers of biopharmaceuticals for myocardial delivery, supporting the development of further research on these delivery systems for heart disease. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Medicina Regenerativa. Rio de Janeiro, RJ, Brasil / Instituto D'Or de Pesquisa e Educação. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Senai de Inovação em Sistemas Avançados de Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Laboratório de Imunopatologia Keizo Asami. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / Universidade de Pernambuco. Faculdade de Ciências Médicas. Recife, PE, Brasil. | pt_BR |
| Subject | Myocardial Infarction | pt_BR |
| Subject | Liposomes | pt_BR |
| Subject | Cardiac Conduction System Disease | pt_BR |
| Subject in Spanish | Infarto del Miocardio | pt_BR |
| Subject in Spanish | Liposomas | pt_BR |
| Subject in Spanish | Trastorno del Sistema de Conducción Cardíaco | pt_BR |
| Subject in French | Infarctus du myocarde | pt_BR |
| Subject in French | Liposomes | pt_BR |
| Subject in French | Trouble de la conduction cardiaque | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
