| Author | Almeida, R. M. | |
| Author | Junqueira, R. M. | |
| Author | Panzenhagen, P. H. N. | |
| Author | Conte Júnior, C. A. | |
| Author | Pereira, R. C. L. | |
| Author | Reis, R. C. L. | |
| Author | Hofer, C. B. | |
| Author | Vallim, D. C. | |
| Access date | 2022-04-27T13:01:28Z | |
| Available date | 2022-04-27T13:01:28Z | |
| Document date | 2021 | |
| Citation | ALMEIDA, R. M. et al. Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples. In: SEMINÁRIO INTERNACIONAL DO PROGRAMA DE PÓS-GRADUAÇÃO EM DOENÇAS INFECCIOSAS E PARASITÁRIAS, 2., 2021, Rio de Janeiro. Cadernos de Resumos... Rio de Janeiro: PPGDIC/Faculdade de Medicina/UFRJ, 2021. 9 p. | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/52427 | |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Listeriose | pt_BR |
| Subject in Portuguese | Listeria Monocytogenes | pt_BR |
| Title | Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples | pt_BR |
| Type | Papers presented at events | |
| Abstract | Introduction: Listeriosis is a serious foodborne illness in high-risk groups such as the elderly, newborns and pregnant women. However, the ecology of Listeria monocytogenes is poorly understood and some strains capacity to adapt to environmental conditions, what makes Lm a major challenge to food safety in the industry. Objective: The aim of this study was to analyze, by Whole Genome Sequencing (WGS), 48 strains of Lm isolated from food (21) and clinical samples (27), from 1975 to 2014 and different States. Method: In silico analysis was performed using tools built in the BIGSdb-Lm database of the Institute Pasteur/France. Result: MLST analysis showed the presence of Clonal Complexes: CC3 (15), CC1 (13), CC218 (9), CC9 (5), CC2 (2), CC7 (1), CC59 (1), CC155 (1) and CC315 (1), corresponding to ST3 (14), ST1 (13), ST218 (9), ST9 (4), ST2 (2), ST7 (1), ST59 (1), ST122 (1), ST520 (1), ST1434 (1) and ST1435 (1) respectively. In addition, two new STs were described, ST1434 and ST1435 isolated from ricotta cheese and human blood, respectively. ST1 was associated with hypervirulence and tropism towards the central nervous system (CNS) and ST9 was strongly associated with food but not clinical samples. Plasmids were found in 23 strains, six of them with the bcrA gene, which confers resistance to benzylkonium chloride. In the chromosome, genes related to resistance against heavy metals (mco (6), mdrL/yfmO (27), merR1 (5), merR2 (5)) and antibiotics (aacA4 (5), fosX (48), sul (46), mprF (48), mdrL/yfmO (27), norB (48), lde (48)) were also detected. Conclusion: WGS allowed a deeper analysis of the genetic diversity of Lm circulating in Brazil, contributing to a better understanding of its virulence and resistance patterns. The sequences are still being analyzed, so patterns and proportions observed so far may be subject to change. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Bras | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Bras | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | WGS | pt_BR |
| Subject | Listeria Monocytogenes | pt_BR |
| Subject | Clonal Complexes | pt_BR |
| Subject | One Health | pt_BR |
