| Author | Picchi-Constante, Gisele Fernanda Assine | |
| Author | Hiraiwa, Priscila Mazzocchi | |
| Author | Marek, Martin | |
| Author | Rogerio, Vanessa Zulkievicz | |
| Author | Guerra-Slompo, Eloise Pavão | |
| Author | Romier, Christophe | |
| Author | Zanchin, Nilson Ivo Tonin | |
| Access date | 2022-05-26T12:04:46Z | |
| Available date | 2022-05-26T12:04:46Z | |
| Document date | 2022 | |
| Citation | PICCHI-CONSTANTE, Gisele Fernanda Assine et al. Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi. Star Protocols, v. 3, n. 101324, p. 1–26, 2022. | pt_BR |
| ISSN | 2666-1667 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/52921 | |
| Language | por | pt_BR |
| Publisher | Cell Press | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Title | Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.xpro.2022.101324 | |
| Abstract | This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleavage, which enables detailed investigation of protein function. This experimental strategy overcomes some of the limitations associated with gene knockouts in T. cruzi. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021). | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Illkirch, France. / Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Department of Integrated Structural Biology. Illkirch, France. / Loschmidt Laboratories. Department of Experimental Biology & RECETOX. Faculty of Science. Masaryk University. Brno, Czech Republic. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Illkirch, France. / Université de Strasbourg, CNRS, INSERM. Institut de Génétique et de Biologie Moléculaire et Cellulaire. Department of Integrated Structural Biology. Illkirch, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Subject | Gene Expression | pt_BR |
| Subject | CRISPR-Associated Protein 9 | pt_BR |
| Subject | Protocols | pt_BR |
| Subject | Gene Editing | pt_BR |
| Subject in Spanish | Expresión Génica | pt_BR |
| Subject in Spanish | Proteína 9 Asociada a CRISPR | pt_BR |
| Subject in Spanish | Edición Génica | pt_BR |
| Subject in French | Expression des gènes | pt_BR |
| Subject in French | Protéine-9 associée à CRISPR | pt_BR |
| Subject in French | Protocoles | pt_BR |
| Subject in French | Édition de gène | pt_BR |
| DeCS | Expressão Gênica | pt_BR |
| DeCS | Proteína 9 Associada à CRISPR | pt_BR |
| DeCS | Protocolos | pt_BR |
| DeCS | Edição de Genes | pt_BR |
