| Author | Villela, Gilberto | |
| Author | Affonso, Ottilia R. | |
| Author | Ribeiro, Luiz P. | |
| Author | Mitidieri, Emilio | |
| Access date | 2022-06-07T18:31:12Z | |
| Available date | 2022-06-07T18:31:12Z | |
| Document date | 1955 | |
| Citation | VILLELA,Gilberto G. et al. Distribuição da xantina oxidase no fígado e no sôro de rato.Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 53, n.2,3,4, p. 1 - 9, 1955. | pt_BR |
| ISSN | 0074-0206 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/53112 | |
| Language | por | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Xantina oxidase | pt_BR |
| Subject in Portuguese | Fígado | pt_BR |
| Subject in Portuguese | Soro de rato | pt_BR |
| Title | Distribuição da xantina oxidase no fígado e no sôro de rato | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761955000200033 | |
| Abstract | The localization of the xanthine oxidase (X.O.) and xanthine dehydrogenase (X.D.) activities in rat liver have been studied using separation of cytoplasmic particles into fractions by differential centrifugation. The results clearly demonstrate that practically all the enzymic activity is present in the supernatant fluid corresponding to the cell sap containing the soluble proteins of the cell. No activity could be detected for the nuclear, mitocondrial and microsomal fractions. The enzymatic activity of the mixture of the four factions was 102 per cent of that of the original homogenate. The distribution of the xanthine dehydrogenase in the protein fractions of the rat serum was accomplished in preliminary experiments by means of 50% ammonium sulphate precipitation and subsequent dialysis against water. All enzymatic activity was confined to the globulin fractions of the serum. Paper electrophoresis was performed and the protein and lipoprotein fractions determined. A method for the localization of the X.D. activity in the protein fractions separated by paper electrophoresis was developed. The results obtained suggest that xanthine dehydrogenase is localized in the globulin fractions possessing mobilities of [alpha 1], [beta] and [gamma] globulins and are probably bound to the lipoproteins. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Xanthine oxidase (X.O.) | pt_BR |
| Subject | xanthine dehydrogenase (X.D.) | pt_BR |
| Subject | Fígado | pt_BR |
| Subject | Soro de rato | pt_BR |
| e-ISSN | 1678-8060 | |
