| Author | Pedrosa, Maria Luysa de Camargos | |
| Author | Oliveira, Alana Karen de | |
| Author | Pereira, Caroline Stephane Salviano | |
| Author | Corsini, Camila Amormino | |
| Author | Teixeira, Sueleny Silva Ferreira | |
| Author | Oliveira, Aureo Almeida de | |
| Author | Katz, Naftale | |
| Author | Salawu, Oyetunde Timothy | |
| Author | Queiroz, Rafaella Fortini Grenfell e | |
| Access date | 2022-06-08T17:28:43Z | |
| Available date | 2022-06-08T17:28:43Z | |
| Document date | 2021 | |
| Citation | PEDROSA, Maria Luysa de Camargos et al. Dot blot platform as a novel diagnostic kit: rapid, accurate, and on-site detection of Schistosoma mansoni in urine samples of hard to detect individuals. Parasitol Res., v. 120, n. 11, 3795-3803, 2021. doi: 10.1007/s00436-021-07312-7 | pt_BR |
| ISSN | 0932-0113 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/53124 | |
| Language | eng | pt_BR |
| Publisher | Springer International | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Dot blot platform as a novel diagnostic kit: rapid, accurate, and on-site detection of Schistosoma mansoni in urine samples of hard to detect individuals | pt_BR |
| Type | Article | |
| DOI | 10.1007/s00436-021-07312-7 | |
| Abstract | Rapid diagnostics provide actionable information for patient care at the time and site of an encounter with the health care system. The mainstay of infectious diseases care is early detection (case finding) and treatment completion, but for many, it is hard to identify positive individuals, as is the case of infection with low burden in schistosomiasis, a parasitic disease common in the tropics and subtropics. We developed a new, accurate, and fast Dot blot methodology (iDot) to indirectly detect Schistosoma mansoni in individuals with very low parasite burden using urine samples. Accuracy of 0.74 was obtained with a significant difference between negative and positive patients and a substantial agreement was found when iDot was compared with five available methods. Our analysis also revealed the superiority of iDot in detecting negative individuals from non-endemic sites, thus, presenting the lowest rate of false positives. This new method called iDot is convenient and suitable for qualitative and quantitative detection of schistosomiasis in individuals with low parasite burden. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation., Belo Horizonte, MG, Brazil/National Reference Center for the Diagnosis of Schistosomiasis. Rene Rachou Institute. Oswaldo Cruz Foundation. Brazilian Ministry of Health. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation., Belo Horizonte, MG, Brazil/National Reference Center for the Diagnosis of Schistosomiasis. Rene Rachou Institute. Oswaldo Cruz Foundation. Brazilian Ministry of Health. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/National Reference Center for the Diagnosis of Schistosomiasis. Rene Rachou Institute. Oswaldo Cruz Foundation. Brazilian Ministry of Health. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Department of Biological Sciences. University of Medical Sciences. Ondo, Ondo State, Nigeria. | pt_BR |
| Affilliation | Diagnosis and Therapy of Infectious Diseases and Cancer. Rene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/National Reference Center for the Diagnosis of Schistosomiasis. Rene Rachou Institute. Oswaldo Cruz Foundation. Brazilian Ministry of Health. Belo Horizonte, MG, Brazil/Department of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, GA, Greece | pt_BR |
| Subject | Dot blot | pt_BR |
| Subject | Rapid diagnosis | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Urine | pt_BR |
