| Author | Reimann, Mayra M. | |
| Author | Santos, Eduardo Caio Torres | |
| Author | Souza, Celeste S. F. de | |
| Author | Andrade Neto, Valter V. | |
| Author | Jansen, Ana Maria | |
| Author | Brazil, Reginaldo P. | |
| Author | Roque, André Luiz R. | |
| Access date | 2022-06-23T18:38:28Z | |
| Available date | 2022-06-23T18:38:28Z | |
| Document date | 2022 | |
| Citation | REIMANN, Mayra M. et al. Oral and Intragastric: New Routes of Infection by Leishmania braziliensis and Leishmania infantum?. Pathogens, v. 11, 688, p. 1 - 13, June 2022. | pt_BR |
| ISSN | 2076-0817 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/53454 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Infecção experimental | pt_BR |
| Subject in Portuguese | Transmissão oral | pt_BR |
| Subject in Portuguese | Transmissão intragástrica | pt_BR |
| Subject in Portuguese | Hamsters | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Title | Oral and Intragastric: New Routes of Infection by Leishmania braziliensis and Leishmania infantum? | pt_BR |
| Type | Article | |
| DOI | 10.3390/ pathogens11060688 | |
| Abstract | Although Leishmania transmission in nature is associated with the bite of an infected
sandfly vector, other possible transmission routes are speculated to occur, such as the oral route. We
evaluated the possibility of infection by this route in golden hamsters (Mesocricetus auratus) using
Leishmania braziliensis (Lb) and Leishmania infantum (Li). Hamsters were exposed to experimental
oral or intragastrical infection with axenic promastigotes, besides oral ingestion of a suspension of
cultivated macrophages infected with amastigotes, lesion-fed Lutzomyia longipalpis, skin lesion or
infective spleen fragment. The parasite’s isolation, besides a positive PCR and IFAT, confirmed the
intragastric infection by promastigote parasites. The oral ingestion of macrophages infected with
L. braziliensis amastigotes was also infective. These results confirmed that Leishmania parasites could
infect mammals by the intragastric route through the ingestion of promastigote forms (what can
happen after a sandfly ingestion) and by the oral ingestion of infected macrophages (what can happen
in nature in a predator–prey interaction). The better understanding of these alternative routes is
essential to understand their transmission dynamics in nature. As far as we know, this is the first time
that oral and intragastric Leishmania transmission has been experimentally demonstrated, constituting
new infection routes, at least for L. infantum and L. braziliensis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Experimental infection | pt_BR |
| Subject | Oral transmission | pt_BR |
| Subject | Intragastric transmission | pt_BR |
| Subject | Hamsters | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Leishmania infantum | pt_BR |
