| Author | Latorre-Estivalis, Jose Manuel | |
| Author | Almeida, Francisca C | |
| Author | Pontes, Gina | |
| Author | Dopazo, Hernán | |
| Author | Barrozo, Romina B | |
| Author | Lorenzo, Marcelo Gustavo | |
| Access date | 2022-06-27T16:39:58Z | |
| Available date | 2022-06-27T16:39:58Z | |
| Document date | 2021 | |
| Citation | LATORRE-ESTIVALIS, Jose Manuel et al. Evolution of the Insect PPK Gene Family. Genome Biol Evol., v. 13, n. 9, evab185, 2021. doi: 10.1093/gbe/evab185. | pt_BR |
| ISSN | 1759-6653 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/53523 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | open access | pt_BR |
| Title | Evolution of the Insect PPK Gene Family | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1093/gbe/evab185 | |
| Abstract | Insect pickpocket (PPK) receptors mediate diverse functions, among them the detection of mechano- and chemo-sensory stimuli. Notwithstanding their relevance, studies on their evolution only focused on Drosophila. We have analyzed the genomes of 26 species of eight orders including holometabolous and hemimetabolous insects (Blattodea, Orthoptera, Hemiptera, Phthiraptera, Hymenoptera, Lepidoptera, Coleoptera, and Diptera), to characterize the evolution of this gene family. PPKs were detected in all genomes analyzed, with 578 genes distributed in seven subfamilies. According to our phylogeny, ppk17 is the most divergent member, composing the new subfamily VII. PPKs evolved under a gene birth-and-death model that generated lineage-specific expansions usually located in clusters, while purifying selection affected several orthogroups. Subfamily V was the largest, including a mosquito-specific expansion that can be considered a new target for pest control. PPKs present a high gene turnover generating considerable variation. On one hand, Musca domestica (59), Aedes albopictus (51), Culex quinquefasciatus (48), and Blattella germanica (41) presented the largest PPK repertoires. On the other hand, Pediculus humanus (only ppk17), bees, and ants (6-9) had the smallest PPK sets. A subset of prevalent PPKs was identified, indicating very conserved functions for these receptors. Finally, at least 20% of the sequences presented calmodulin-binding motifs, suggesting that these PPKs may amplify sensory responses similarly as proposed for Drosophila melanogaster ppk25. Overall, this work characterized the evolutionary history of these receptors revealing relevant unknown gene sequence features and clade-specific expansions. | pt_BR |
| Affilliation | Laboratorio de Insectos Sociales. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Grupo de Investigación en Filogeografía y Filogenias Moleculares. Departamento de Ecología. Genética y Evolución. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Laboratorio de Eco-Fisiología de Insectos del Instituto de Biodiversidad y Biología Experimental y Aplicada. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina. | pt_BR |
| Affilliation | Laboratorio de Genómica de Poblaciones y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Grupo de Neuroetología de Insectos Vectores. Laboratorio de Fisiología de Insectos. Instituto de Biodiversidad y Biología Experimental y Aplicada. Departamento de Biología y Biodiversidad Experimental. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Buenos Aires, Argentina. | pt_BR |
| Affilliation | Vector Behaviour and Pathogen Interaction Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. | pt_BR |
| Subject | DEG/ENaCs | pt_BR |
| Subject | evolution | pt_BR |
| Subject | insects | pt_BR |
| Subject | phylogeny | pt_BR |
| Subject | pickpocket receptors | pt_BR |
