| Author | Rodrigues, Bruno Leite | |
| Author | Costa, Glaucilene da Silva | |
| Author | Shimabukuro, Paloma Helena Fernandes | |
| Access date | 2022-06-29T18:06:12Z | |
| Available date | 2022-06-29T18:06:12Z | |
| Document date | 2021 | |
| Citation | RODRIGUES, Bruno Leite; COSTA, Glaucilene da Silva; SHIMABUKURO, Paloma Helena Fernandes. Identification of Bloodmeals from Sand Flies (Diptera: Psychodidae) Collected in the Parque Nacional do Viruá, State of Roraima, Brazil. J Med Entomol., v. 58, n. 6, p. 2488-2494, 2021. doi: 10.1093/jme/tjab068. | pt_BR |
| ISSN | 0022-2585 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/53581 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Identification of Bloodmeals from Sand Flies (Diptera: Psychodidae) Collected in the Parque Nacional do Viruá, State of Roraima, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1093/jme/tjab068 | |
| Abstract | The transmission of pathogens that cause leishmaniases occurs by the bite of female sand flies (Diptera: Psychodidae) in their vertebrate hosts, which makes the identification of their bloodmeal sources an important step for the control and epidemiology of these diseases. In Brazil, the state of Roraima has a great diversity of sand flies, vertebrate hosts, and protozoan Leishmania, but little is known about the host blood-feeding preferences of sand flies. Thus, we evaluated the bloodmeal sources of sand flies collected from their sylvatic habitats in Parque Nacional do Viruá, Roraima. Fieldwork was carried-out between 13th and 18th August 2019 using CDC light traps. Sand flies were slide-mounted and morphologically identified using the head and last segments of the abdomen. Engorged females had their DNA extracted, followed by amplification and sequencing of the cytochrome b (cytb) molecular marker for vertebrates. Sequences were analyzed and compared with those from GenBank using the BLASTn search tool, in addition to the reconstruction of a phylogenetic tree to demonstrate the clustering pattern of these sequences. A total of 1,209 sand flies were identified, comprising 20 species, in which the most abundant were Psychodopygus ayrozai (Barretto and Coutinho) (42.10%) and Psychodopygus chagasi (Costa Lima) (26.22%). Bloodmeal source identification was successfully performed for 34 sand flies, that confirm four vertebrate species, being the most abundant the armadillo Dasypus novemcinctus Linnaeus, 1758 (Cingulata: Dasypodidae). | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Laboratório de Entomologia. Porto Velho, RO, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Instituto René Rachou. Coleção de Flebotomíneos. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | bloodmeal identification | pt_BR |
| Subject | host preference | pt_BR |
| Subject | leishmaniases | pt_BR |
| Subject | molecular identification | pt_BR |
| Subject | transmission | pt_BR |
| Subject | vector | pt_BR |
