| Author | Dario, Maria Augusta | |
| Author | Lisboa, Cristiane Varella | |
| Author | Xavier, Samanta Cristina das Chagas | |
| Author | D`Andrea, Paulo Sérgio | |
| Author | Roque, André Luiz Rodrigues | |
| Author | Jansen, Ana Maria | |
| Access date | 2022-07-23T12:14:32Z | |
| Available date | 2022-07-23T12:14:32Z | |
| Document date | 2022 | |
| Citation | DARIO, Maria Augusta et al. Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case. Frontiers in Cellular and Infection Microbiology, v. 12, Article 812708, p. 1 - 12, Feb. 2022. | pt_BR |
| ISSN | 2235-2988 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/53925 | |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosomatidae | pt_BR |
| Subject in Portuguese | Hospedeiro mamífero | pt_BR |
| Subject in Portuguese | Clado Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Mata Atlântica | pt_BR |
| Title | Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case | pt_BR |
| Type | Article | |
| DOI | 10.3389/fcimb.2022.812708 | |
| Abstract | Trypanosomatids are hemoflagellate parasites that even though they have been increasingly
studied, many aspects of their biology and taxonomy remain unknown. The aimof this study
was to investigate the Trypanosoma sp. transmission cycle in nonflying small mammals in
an area where a case of acute Chagas disease occurred in Mangaratibamunicipality, Rio de
Janeiro state. Three expeditions were conducted in the area: the first in 2012, soon after the
human case, and two others in 2015. Sylvatic mammals were captured and submitted to
blood collection for trypanosomatid parasitological and serological exams. Dogs from the
surrounding areas where the sylvatic mammals were captured were also tested for T. cruzi
infection. DNA samples were extracted from blood clots and positive hemocultures,
submitted to polymerase chain reaction targeting SSU rDNA and gGAPDH genes,
sequenced and phylogenetic analysed. Twenty-one wild mammals were captured in
2012, mainly rodents, and 17 mammals, mainly marsupials, were captured in the two
expeditions conducted in 2015. Only four rodents demonstrated borderline serological
T. cruzi test (IFAT), two in 2012 and two in 2015. Trypanosoma janseni was the main
Trypanosoma species identified, and isolates were obtained solely from Didelphis aurita. In
addition to biological differences, molecular differences are suggestive of genetic diversity
in this flagellate species. Trypanosoma sp. DID was identified in blood clots from D. aurita in
single and mixed infections with T. janseni. Concerning dogs, 12 presented mostly
borderline serological titers for T. cruzi and no positive hemoculture. In blood clots from
11 dogs, T. cruzi DNA was detected and characterized as TcI (n = 9) or TcII (n = 2).
Infections by Trypanosoma rangeli lineage E (n = 2) and, for the first time, Trypanosoma
caninum, Trypanosoma dionisii, and Crithidia mellificae (n = 1 each) were also detected in
dogs. We concluded that despite the low mammalian species richness and degraded
environment, a high Trypanosoma species richness species was being transmitted with the
predominance of T. janseni and not T. cruzi, as would be expected in a locality of an acute
case of Chagas disease. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosomatidae | pt_BR |
| Subject | Mammalian host | pt_BR |
| Subject | Trypanosoma cruzi clade | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Atlantic Forest | pt_BR |
| xmlui.metadata.dc.subject.ods | 15 Vida terrestre | |
