| Autor | Marcato, L. G. | |
| Autor | Ferlini, Aline Peres | |
| Autor | Bonfim, R. C. F. | |
| Autor | Ramos-Jorge, Maria Letícia | |
| Autor | Ropert, Catherine Maryveite | |
| Autor | Afonso, Luis Fernando Crocco | |
| Autor | Vieira, Leda Q. | |
| Autor | Ribeiro Sobrinho, Antônio Paulino | |
| Data de acesso | 2022-07-29T14:10:04Z | |
| Data de disponibilização | 2022-07-29T14:10:04Z | |
| Data do publicação | 2008 | |
| Citação | MARCATO, L. G. et al. The role of Toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens. Oral Microbiol Immunol., v. 23, n. 5, p. 353-359, 2008. doi: 10.1111/j.1399-302X.2008.00432.x. | pt_BR |
| ISSN | 0902-0055 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54064 | |
| Fomento | COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR | pt_BR |
| Idioma | eng | pt_BR |
| Editor | Munksgaard | pt_BR |
| Direito Autoral | restricted access | pt_BR |
| Título | The role of Toll-like receptors 2 and 4 on reactive oxygen species and nitric oxide production by macrophage cells stimulated with root canal pathogens | pt_BR |
| Tipo do documento | Article | |
| DOI | 10.1111/j.1399-302X.2008.00432.x | |
| Resumo em Inglês | Introduction: Periapical lesions arise as a result of the activation and interaction of the host immune responses against root canal infection. Recently identified Toll-like receptors (TLR) seem to be involved in the recognition and development of immune responses against a myriad of microorganisms. However, very little information is available on the role of TLR in the induction of periapical lesions. Method: The role of TLR-2 and TLR-4 in the activation of murine macrophages stimulated using Fusobacterium nucleatum and Peptostreptococcus anaerobius was investigated. The production of nitric oxide (NO) and reactive oxygen species (ROS) was assessed. Results: The results demonstrate that TLR-2 and TLR-4 are involved in the production of ROS by activated macrophages. The microorganisms induced similar levels of NO production by TLR-2-competent and TLR-2-deficient macrophages, regardless of the addition of interferon-gamma (IFN-gamma), ruling out a role for TLR-2 in the NO production induced by these bacteria. Only P. anaerobius induced NO production by TLR-4-competent macrophages without the addition of IFN-gamma. However, after IFN-gamma addition, F. nucleatum induced macrophage NO production. Therefore, NO production stimulated by IFN-gamma and these microorganisms seems to be TLR-4-independent. Conclusion: TLR-2 seems to be involved in the induction of ROS production by macrophages in response to prevalent root canal bacteria, while only F. nucleatum induced ROS production by TLR-4-competent macrophages. Both microorganisms significantly induced large amounts of NO independent of TLR-2 and TLR-4. We conclude that microorganisms may participate in the induction and progression of periapical lesions through NO and ROS production by activated macrophages | pt_BR |
| Afiliação | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Rene Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Department of Mathematics. Institute of Exact Sciences. Universidade Federal de Juiz de Fora. MG, Brazil | pt_BR |
| Afiliação | Department of Biochemistry and Immunology. Institute of Biological Sciences. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Department of Restorative Dentistry. School of Dentistry. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Palavras-chave em inglês | innate immunity | pt_BR |
| Palavras-chave em inglês | nitric oxide | pt_BR |
| Palavras-chave em inglês | reactive oxygen species | pt_BR |
| Palavras-chave em inglês | root canal infection | pt_BR |
| Palavras-chave em inglês | Toll-like receptors | pt_BR |
