| Author | Macena, Lorena da Graça Pedrosa de | |
| Author | Pereira, Joseane Simone de Oliveira | |
| Author | Silva, Jansen Couto da | |
| Author | Ferreira, Fernando César | |
| Author | Maranhão, Adriana Gonçalves | |
| Author | Lanzarini, Natália Maria | |
| Author | Miagostovic, Marize Pereira | |
| Access date | 2022-07-31T13:16:11Z | |
| Available date | 2022-07-31T13:16:11Z | |
| Document date | 2022 | |
| Citation | MACENA, Lorena da Graça Pedrosa de et al. Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx‑qPCR. Brazilian Journal of Microbiology, on line, p. 1 - 7, May 2022. | pt_BR |
| ISSN | 1678-4405 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54090 | |
| Language | eng | pt_BR |
| Publisher | Sociedade Brasileira de Microbiologia | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Monoazida de propídio | pt_BR |
| Subject in Portuguese | PMAxx-qPCR | pt_BR |
| Subject in Portuguese | Esgoto bruto | pt_BR |
| Subject in Portuguese | Àgua salobra | pt_BR |
| Subject in Portuguese | Água do mar | pt_BR |
| Subject in Portuguese | HAdV | pt_BR |
| Title | Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx‑qPCR | pt_BR |
| Type | Article | |
| DOI | 10.1007/s42770-022-00775-5 | |
| Abstract | Molecular methodologies providing data on viral concentration and infectivity have been successfully used in environmental
virology, supporting quantitative risk assessment studies. The present study aimed to assess human mastadenovirus (HAdV)
intact particles using a derivative of propidium monoazide associated with qPCR (PMAxx-qPCR) in aquatic matrices.
Initially, different concentrations of PMAxx were evaluated to establish an optimal protocol for treating different naturally
contaminated matrices, using 10 min incubation in the dark at 200 rpm at room temperature and 15 min of photoactivation
in the PMA-Lite™ LED photolysis device. There was no significant reduction in the quantification of infectious HAdV
with increasing concentration of PMAxx used (20 μM, 50 μM, and 100 μM), except for sewage samples. In this matrix, a
reduction of 5.01 log of genomic copies (GC)/L was observed from the concentration of 50 μM and revealed 100% HAdV
particles with damaged capsids. On the other hand, the mean reduction of 0.51 log in stool samples using the same concentration
mentioned above demonstrated 83% of damaged particles eliminated in the stool. Following, 50 μM PMAxx-qPCR
protocol revealed a log reduction of 0.91, 0.67, and 1.05 in other samples of raw sewage, brackish, and seawater where HAdV
concentration reached 1.47 × 104,
6.81 × 102,
and 2.33 × 102
GC/L, respectively. Fifty micrometers of PMAxx protocol
helped screen intact viruses from different matrices, including sea and brackish water. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Propidium monoazide | pt_BR |
| Subject | PMAxx-qPCR | pt_BR |
| Subject | HAdV | pt_BR |
| Subject | Raw sewage | pt_BR |
| Subject | Brackish water | pt_BR |
| Subject | Seawater | pt_BR |
| xmlui.metadata.dc.subject.ods | 06 Água potável e saneamento | |
