| Author | Pereira, Cristiane Bigatti | |
| Author | Sá, Nívea Pereira de | |
| Author | Borelli, Beatriz Martins | |
| Author | Rosa, Carlos Augusto | |
| Author | Barbeira, Paulo Jorge Sanches | |
| Author | Cota, Betania Barros | |
| Author | Johann, Susana | |
| Access date | 2022-08-01T16:31:16Z | |
| Available date | 2022-08-01T16:31:16Z | |
| Document date | 2016 | |
| Citation | PEREIRA, Cristiane Bigatti et al. Antifungal activity of eicosanoic acids isolated from the endophytic fungus Mycosphaerella sp. against Cryptococcus neoformans and C. gattii. Microb Pathog., v. 100, p. 205-212, 2016. doi: 10.1016/j.micpath.2016.09.022. | pt_BR |
| ISSN | 0882-4010 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54111 | |
| Sponsorship | CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO | pt_BR |
| Language | eng | pt_BR |
| Publisher | Academic Press | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Antifungal activity of eicosanoic acids isolated from the endophytic fungus Mycosphaerella sp against Cryptococcus neoformans and C-gattii | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.micpath.2016.09.022 | |
| Abstract | The antifungal effects of two eicosanoic acids, 2-amino-3,4-dihydroxy-2-25-(hydroxymethyl)-14-oxo-6,12-eicosenoic acid (compound 1) and myriocin (compound 2), isolated from Mycosphaerella sp. were evaluated against Cryptococcus neoformans and C gattii. The compounds displayed antifungal activities against several isolates of C neoformans and C gattii, with minimal inhibitory concentration (MIC) values ranging from 0.49 to 7.82 mu M for compound 1 and 0.48-1.95 mu M for compound 2. In the checkerboard microtiter test, both compounds exhibited synergistic activity with amphotericin B against C gattii. Ultrastructural analysis revealed several signs of damage in C. gattii and C. neoformans cells treated with compounds 1 and 2, including deformities in cell shape, depressions on the surface, and withered cells. The cells of C gattii treated with compounds 1 and 2 showed less loss of cellular material in comparison to those treated with amphotericin B. The difference in cellular material loss increased in a test compound concentration-dependent manner. Consistent with this observation, compounds 1 and 2 were able to internalize propidium iodide (PI) in C gattii cells. In addition, compound 2 induced the formation of several pseudohyphae, suggesting that it could reduce virulence in C gattii cells. The study results show that these natural products led to membrane damage; however, this may not be the main target of action. These compounds have potential antifungal activity and could be useful in further studies for developing more effective combination therapies with amphotericin B and reducing side effects in patients. (C) 2016 Elsevier Ltd. All rights reserved. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Química. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Cryptococcus | pt_BR |
| Subject | Membrane damage | pt_BR |
| Subject | Mycosphaerella | pt_BR |
| Subject | Synergism. | pt_BR |
