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| Autor | Pissinate, Jauber Fornaciari | |
| Autor | Gomes, Izabelle Teixeira | |
| Autor | Paschoal, Vanessa Peruhype Magalhães | |
| Autor | Dietze, Reynaldo | |
| Autor | Martins Filho, Olindo Assis | |
| Autor | Lemos, Elenice Moreira | |
| Fecha de acceso | 2022-08-01T19:06:29Z | |
| Fecha de disponibilización | 2022-08-01T19:06:29Z | |
| Fecha de publicación | 2008 | |
| Referencia | PISSINATE, Jauber Fornaciari et al. Upgrading the flow-cytometric analysis of anti-Leishmania immunoglobulins for the diagnosis of American tegumentary leishmaniasis. J Immunol Methods, v. 336, n. 2, p. 193-202, 2008. doi: 10.1016/j.jim.2008.04.018. | pt_BR |
| ISSN | 0022-1759 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54189 | |
| Promoción | FUNDAÇÃO DE AMPARO À PESQUISA E INOVAÇÃO DO ESPIRITO SANTO | pt_BR |
| Idioma | eng | pt_BR |
| Editor | Elsevier | pt_BR |
| Derechos de autor | restricted access | pt_BR |
| Título | Upgrading the flow-cytometric analysis of anti-Leishmania immunoglobulins for the diagnosis of American tegumentary leishmaniasis | pt_BR |
| Tipo del documento | Article | |
| DOI | 10.1016/j.jim.2008.04.018 | |
| Resumen en Inglés | We have previously described a flow cytometry-based assay to detect anti-live Leishmania (Viania) braziliensis promastigote antibodies (FC-ALPA) with prominent performance of FC-ALPA to diagnosis American tegumentary leishmaniasis (ATL). However, the laboriousness to work with live parasites represented the major drawback for using FC-ALPA in routine clinical laboratory. Herein, we have presented an upgraded technology using fixed Leishmania (Leishmania) amazonensis promastigotes as antigen (FC-AFPA). Our data demonstrated that FC-AFPA-IgG displays outstanding performance for ATL diagnosis with high sensitivity (99%) and specificity (100%). Moreover, Likelihood Ratio indicated that positive results (LR+) has an infinite times more chance to come from ATL than from non-infected individuals (NI). Despite the high frequency of cross-reactivity with putative ATL co-endemic diseases, including visceral leishmaniasis, Chagas disease and malaria, FC-AFPA-IgG showed remarkable potential for differential diagnosis with other dermatological illnesses such as leprosy and sporotrichosis. FC-AFPA-IgG subclasses analysis revealed that LTA is characterized by IgG1 > IgG3 > IgG2 = IgG4 anti-L. amazonensis profiling, electing FC-AFPA-IgG1 and IgG3 with better performances to diagnosis ATL diagnosis. Additionally, FC-AFPA-IgG3 showed to be a better diagnostic tool in endemic areas for malarial disease. Despite the substantial advance to work with fixed promastigotes that contributes to its higher sensitivity, the lower specificity of FC-AFPA represented the major flaws as compared to FC-ALPA, suggesting that further improvement is still required to minimize the cross-reactivity with trypanosomatidae infections. Perspectives for using a flow cytometry multiplex based methodology to simultaneously assess anti-L. amazonensis, anti-L. chagasi and anti-Trypanosoma cruzi IgG reactivity is currently under investigation. (C) 2008 Elsevier B.V. All rights reserved | pt_BR |
| Afiliación | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brazil | pt_BR |
| Afiliación | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brazil | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brazil | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliación | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brazil | pt_BR |
| Palavras clave en Inglês | flow cytometry | pt_BR |
| Palavras clave en Inglês | American tegumentary leishmaniasis | pt_BR |
| Palavras clave en Inglês | serologic diagnosis | pt_BR |
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