| Author | Silva, Luciana Maria | |
| Author | Lages, Carolina Pereira | |
| Author | Venuto, Thiago | |
| Author | Lima, Rebeca Mascarenhas | |
| Author | Diniz, Marcelo Vasconcellos | |
| Author | Valentim, Claúdia L. Lage | |
| Author | Baba | |
| Author | Pimenta, Paulo Filemon Paolucci | |
| Author | Fortes-Dias, Consuelo Latorre | |
| Access date | 2022-08-09T17:07:30Z | |
| Available date | 2022-08-09T17:07:30Z | |
| Document date | 2008 | |
| Citation | SILVA, Luciana Maria et al. Primary culture of venom glands from the Brazilian armed spider, Phoneutria nigriventer (Araneae, Ctenidae). Toxicon, v. 51, n. 3, p. 428-434, 2008. doi: 10.1016/j.toxicon.2007.10.018. | pt_BR |
| ISSN | 0041-0101 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54540 | |
| Sponsorship | CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO | pt_BR |
| Language | eng | pt_BR |
| Publisher | Pergamon Press | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Primary culture of venom glands from the Brazilian armed spider, Phoneutria nigriventer (Araneae, Ctenidae) | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.toxicon.2007.10.018 | |
| Abstract | Phoneutria spider venoms are a rich source of bioactive components. The limited amounts of crude material available, however, can be considered as a major hindrance for a faster development in the field. In the present study, we attempted to establish primary cultures of venom glands of Phoneutria nigriventer as an alternative, in vitro source of venom. Three different developmental stages were tried as starting materials: whole embryo (inside the cocoon), nymph (early after cocoon hatching) and young adult (I year after cocoon hatching). The embryonic cells remained in suspension in the primary cultures, with no signs of adhesion or differentiation, for about 6 months. Nevertheless, this culture was useful for the first chromosome C-banding of Phoneutria. An average of 29 +/- 1 acrocentric chromosomes were found. Striated muscle cells were the only kind of cells in the culture of venom glands from Phoneutria nymphs. The most promising results were achieved with 1-year-old specimens. Besides muscle, adherent epithelial cells were also obtained in culture. Although these cells remained in culture for a short time (up to 48 h) immunochemical analysis of the culture supernatant evidenced the presence of Phoneutria venom components. This can be considered as a first step toward the functional cultures of venom glands of Phoneutria spiders. (c) 2007 Elsevier Ltd. All rights reserved | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene ́ Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Departamento de Ciências Biologicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Departamento de Ciências Biologicas. Ouro Preto, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Ezequiel Dias. Diretoria de Pesquisa e Desenvolvimento. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Phoneutria | pt_BR |
| Subject | Phoneutria nigriventer | pt_BR |
| Subject | armed spider | pt_BR |
| Subject | banana spider | pt_BR |
| Subject | tissue culture | pt_BR |
| Subject | venom gland | pt_BR |
