| Author | Malheiro, Adriana | |
| Author | Anıbal, Fernanda de Freitas | |
| Author | Martins Filho, Olindo Assis | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Perini, Adenir | |
| Author | Martins, Milton Arruda | |
| Author | Medeiros, Alexandra Ivo de | |
| Author | Turato, Walter Miguel | |
| Author | Acencio, Milene Marques Pagliarelli | |
| Author | Brandão, Izaíra Tincani | |
| Author | Nomizo, Auro | |
| Author | Silva, Celio L. | |
| Author | Faccioli, Lúcia Helena | |
| Access date | 2022-08-10T18:57:42Z | |
| Available date | 2022-08-10T18:57:42Z | |
| Document date | 2008 | |
| Citation | MALHEIRO, Adriana et al. pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infection. Vaccine, v. 26, n. 3, p. 305-315, 2008. | pt_BR |
| ISSN | 0264-410X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54573 | |
| Sponsorship | FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier Science | pt_BR |
| Rights | restricted access | pt_BR |
| Title | pcDNA-IL-12 vaccination blocks eosinophilic inflammation but not airway hyperresponsiveness following murine Toxocara canis infection | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vaccine.2007.11.023 | |
| Abstract | We have investigated the effect of pcDNA3-CpG and pcDNA-IL-12, delivered by intradermal gene gun administration, on the blood/lung eosinophilia, airway hyperresponsiveness as well as the immune response in a murine model of toxocariasis. Our results demonstrated that pcDNA-IL-12 but not pcDNA3-CpG vaccination Led to a persistent tower blood/bronchoalveolar eosinophilia following Toxocaro conis infection, as pcDNA3-CpG led only to an early transient blockage of eosinophil transmigration into bronchoalveolar fluid following T canis infection. Prominent Type-1 immune response was pointed out as the halt-mark of T canis infection following pcDNA-IL-12 vaccination. Outstanding IFN-gamma/IL-4 ratio besides tow levels of IgG1 with subsequent high IgG2a/IgG1 ratio further characterized a Type-1 polarized immunological profile in pcDNA-IL-12-vaccinated animals. Nevertheless, only pcDNA3-CpG was able to prevent airway hyperresponsiveness induced by T canis infection. The persistent airway hyperresponsiveness observed in pcDNA-IL-12-vaccinated animals demonstrated that the airway constriction involved other immunological mediator than those blocked by pcDNA-IL-12. Together, these data indicated that pcDNA-IL-12 and pcDNA3-CpG vaccines have distinct therapeutic benefits regarding the eosinophilic inflammation/airway hyperresponsiveness triggered by T canis infection, suggesting their possible use in further combined therapeutic interventions. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil. | pt_BR |
| Affilliation | Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquımica e Imunologia. Universidade de São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquımica e Imunologia. Universidade de São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquımica e Imunologia. Universidade de São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquımica e Imunologia. Universidade de São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquımica e Imunologia. Universidade de São Paulo. Ribeirão Preto, SP, Brazil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brazil. | pt_BR |
| Subject | pcDNA-IL-12 | pt_BR |
| Subject | DNA vaccine | pt_BR |
| Subject | Eosinophilic inflammation | pt_BR |
| Subject | Airway hyperresponsiveness | pt_BR |
| Subject | Toxocariasis | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
