| Author | Campioni, Fábio | |
| Author | Vilela, Felipe Pinheiro | |
| Author | Cao, Guojie | |
| Author | Kastanis, George | |
| Author | Rodrigues, Dália dos Prazeres | |
| Author | Costa, Renata Garcia | |
| Author | Tiba‑Casas, Monique Ribeiro | |
| Author | Yin, Lanlan | |
| Author | Allard, Marc | |
| Author | Falcão, Juliana Pfrimer | |
| Access date | 2022-08-14T17:43:41Z | |
| Available date | 2022-08-14T17:43:41Z | |
| Document date | 2022 | |
| Citation | CAMPIONI, Fábio et al. Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades. Scientific Reports, v. 12, 10555, p. 1 - 6, 2022. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/54632 | |
| Language | eng | pt_BR |
| Publisher | Nature Portfolio | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Sequenciamento completo do genoma | pt_BR |
| Subject in Portuguese | Análises | pt_BR |
| Subject in Portuguese | Revelam | pt_BR |
| Subject in Portuguese | Salmonella cepas enterica sorovar Dublin | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Subject in Portuguese | Pertencia a dois clados predominantes | pt_BR |
| Title | Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-022-14492-4 | |
| Abstract | Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. Dublin
strains isolated in Brazil and in other countries were analyzed to determine their phylogenetic
relationships, the presence of genes, plasmids, genomic regions related to virulence and antimicrobial
resistance genes repertoire, using WGS analyses. Illumina was used to sequence the genome of 112
S. Dublin strains isolated in Brazil from humans (n = 82) and animals (n = 30) between 1983 and 2016.
Furthermore, 87 strains from other countries were analyzed. WGSNP analysis revealed three different
clades, in which the strains from Brazil belonged to two clades, A and C. Most of the genes and
genomic regions searched varied among the strains studied. The siderophore genes iroB and iroC were
exclusively found in strains from Brazil and pegD gene, related to fimbrial adherence determinants,
were positive in 124 strains from clades A and B but absent in all the strains from clade C (n = 71).
Eleven plasmid replicons were found in the strains from Brazil, and nine were exclusively found in
strains from other countries. The antimicrobial resistance genes mdsA and mdsB, that encode an efflux
pump, were found in all the strains studied. The strains from Brazil carried other resistance genes,
such as tet(A) (n = 11), tet(B) (n = 4) and tet(C) (n = 4), blaTEM-1 (n = 4), catA1 (n = 1), aadA1 (n = 1), and
sul1 (n = 1). In conclusion, S. Dublin strains isolated in Brazil presented some few unique genes not
found in strains from other countries and were allocated into two distinct clades with strains of human
and animal origin epidemiologically related. This fact stresses the zoonotic potential of S. Dublin
circulating in Brazil for more than 30 years. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Affilliation | Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA. | pt_BR |
| Affilliation | Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Adolfo Lutz. Centro de Bacteriologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Office of Analytics and Outreach, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, 5001 Campus Drive, College Park, MD, USA. | pt_BR |
| Affilliation | Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil. | pt_BR |
| Subject | Whole genome sequencing | pt_BR |
| Subject | Analyses | pt_BR |
| Subject | Salmonella enterica serovar Dublin | pt_BR |
| Subject | Strains | pt_BR |
| Subject | Two predominant clades | pt_BR |
| Subject | Brazil | pt_BR |
