| Author | Silva, C. L. M. | |
| Author | Lenzi, Henrique Leonel | |
| Author | Silva, V. F. M. | |
| Author | Paulo, F. O. | |
| Author | Noel, F. | |
| Access date | 2012-09-06T14:37:51Z | |
| Available date | 2012-09-06T14:37:51Z | |
| Document date | 2003 | |
| Citation | SILVA, C. L. M. ; LENZI, H. L. ; SILVA, V. F. et al. Cellular mechanisms involved in the increased contration of portal veins from Schistosoma mansoni infected mice. Parasitology Research, v. 89, n. 1, p. 16-22, 2003. | pt_BR |
| ISSN | 0932-0113 | |
| URI | https://www.arca.fiocruz.br/handle/icict/5487 | |
| Sponsorship | Acknowledgements The authors thank Dr. Lygia Paraense (FIOCRUZ, Rio de Janeiro, Brazil) for kindly providing infected mice
and Jose´ Ferreira Oliveira for skilful technical assistance. FUJB (8992-3), FAPERJ and CNPq (Brazil) supported this work. F.N., F.O.P. and V.F.M.S. are recipients of CNPq and FAPERJ fellowships | pt_BR |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | en |
| Rights | open access | |
| Title | Cellular mechanisms involved in the increased contraction of portal veins from Schistosoma mansoni-infected mice | pt_BR |
| Type | Article | |
| DOI | DOI 10.1007/s00436-002-0711-7 | |
| Abstract | We previously reported that portal veins from mice infected with male Schistosoma mansoni exhibited an increased reactivity to 5-hydroxytryptamine (5-HT). Here, we extended our observations to mice infected by both male and female worms and we further investigated another constrictor agent and the mechanism(s) responsible for the enhanced maximal contraction ( E(max)). Bisexual infection increased the E(max) of 5-HT (from 0.66+/-0.06 mN.s to 1.56+/-0.38 mN.s), in a similar way to the unisexual (male) infection. Infection with male worms increased portal vein reactivity to acetylcholine, as revealed by a higher E(max) (1.03+/-0.2 mN.s) in relation to non-infected control animals ( E(max)= 0.54+/-0.08 mN.s). Sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase (SERCA) inhibition with 100 nM thapsigargin reduced the E(max) of 5-HT by 35% in both tissues, discharging a deficiency of SERCA pump in infected animals. In contrast, the number of voltage-dependent Ca(2+) channels (L-type) was higher in portal veins from infected than non-infected control mice. Inhibition of Ca(2+)-activated chloride channels (Cl(Ca)) with 10 micro M niflumic acid reduced the E(max) of 5-HT in portal veins more from infected than non-infected animals (remaining tension = 60.9+/-2.2% and 70.4+/-2.3%, respectively). Histopathological analysis revealed an increased content of collagen and elastin in portal veins from male S. mansoni-infected mice, compatible with an increased intraluminal pressure. In conclusion, male S. mansoni altered portal vein physiology, increasing the E(max) of two vasoconstrictors, possibly by increasing membrane depolarisation through a more effective opening of Cl(Ca) channels, with calcium entering through L-type Ca(2+) channels. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Farmacologia Básica e Clínica. Laboratório de Farmacologia Bioquímica e Molecular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Acetylcholine | pt_BR |
| Subject | Dose-Response Relationship | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | calcium | pt_BR |
| Subject | Serotonin | pt_BR |
| Subject | Sex Factors | pt_BR |
| Subject | Sarcoplasmic Reticulum | pt_BR |
| DeCS | Retículo Sarcoplasmático | pt_BR |
| DeCS | Schistosoma mansoni | pt_BR |
| DeCS | Acetilcolina | pt_BR |
| DeCS | Antagonistas da Serotonina | pt_BR |
| DeCS | Cálcio | pt_BR |
| DeCS | Fatores Sexuais | pt_BR |
| DeCS | Histocitoquímica | pt_BR |
| xmlui.metadata.dc.subject.ods | 05 Igualdade de gênero | |
