| Author | Galvão, Alexandre F. C | |
| Author | Araújo, Morgana de S | |
| Author | Silva, Valdenizia R | |
| Author | Santos, Luciano de S | |
| Author | Dias, Rosane B | |
| Author | Rocha, Clarissa A. Gurgel | |
| Author | Soares, Milena B. P | |
| Author | Silva, Felipe M. A. da | |
| Author | Koolen, Hector H. F | |
| Author | Zengin, Gokhan | |
| Author | Costa, Emmanoel V | |
| Author | Bezerra, Daniel P | |
| Access date | 2022-09-27T17:53:29Z | |
| Available date | 2022-09-27T17:53:29Z | |
| Document date | 2022 | |
| Citation | GALVÃO, Alexandre F. C. et al. Antitumor effect of guatteria olivacea R. E. Fr. (Annonaceae) leaf essential oil in liver cancer. Molecules, v. 27, p. 1-15, 2022. | en_US |
| ISSN | 1420-3049 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/54902 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM).
Fundação Oswaldo Cruz (FIOCRUZ).
Universidade Federal do Amazonas (UFAM). | en_US |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | en_US |
| MeSH | Liver Neoplasms | en_US |
| Subject in Portuguese | Guatteria olivacea | en_US |
| Subject in Portuguese | Óleo essencial | en_US |
| Subject in Portuguese | Câncer de fígado | en_US |
| Subject in Portuguese | Antitumoral | en_US |
| Title | Antitumor effect of guatteria olivacea R. E. Fr. (Annonaceae) leaf essential oil in liver cancer | en_US |
| Type | Article | en_US |
| DOI | 10.3390/ molecules27144407 | |
| Abstract | Guatteria olivacea R. E. Fries (synonym Guatteria punctata (Aubl.) R.A. Howard) is a tree of 10–27 m tall popularly known as “envira-bobó”, “envira-fofa”, “envireira”, “embira”, “embira-branca”, “embira-preta”, envira-branca”, and “envira-preta”, which can be found in the Brazilian Amazon biome. In this study, we evaluated the cytotoxic and antitumor effects of the essential oil (EO) obtained from the leaves of G. olivacea against liver cancer using HepG2 cells as a model. EO was obtained using a hydrodistillation Clevenger-type apparatus and was qualitatively and quantitatively characterized using GC–MS and GC–FID, respectively. The alamar blue assay was used to assess the cytotoxic potential of EO in a panel of human cancer cell lines and human non-cancerous cells. In HepG2 cells treated with EO, YO-PRO-1/propidium iodide staining, cell cycle distribution, and reactive oxygen species (ROS) were examined. In C.B-17 SCID mice with HepG2 cell xenografts, the efficacy of the EO (20 and 40 mg/kg) was tested in vivo. GC–MS and GC–FID analyses showed germacrene D (17.65%), 1-epi-cubenol (13.21%), caryophyllene oxide (12.03%), spathulenol (11.26%), (E)-caryophyllene (7.26%), bicyclogermacrene (5.87%), and δ-elemene (4.95%) as the major constituents of G. olivacea leaf EO. In vitro cytotoxicity of EO was observed, including anti-liver cancer action with an IC50 value of 30.82 μg/mL for HepG2 cells. In HepG2 cells, EO treatment increased apoptotic cells and DNA fragmentation, without changes in ROS levels. Furthermore, the EO inhibited tumor mass in vivo by 32.8–57.9%. These findings suggest that G. olivacea leaf EO has anti-liver cancer potential. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto SENAI de Inovação em Sistemas Avançados de Saúde. SENAI CIMATEC. Salvador, BA, Brasil. | en_US |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | en_US |
| Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Grupo de Pesquisa em Metabolômica e Espectrometria de Massa. Manaus, AM, Brasil. | en_US |
| Affilliation | Department of Biology. Science Faculty. Selcuk University. Konya, Turkey. | en_US |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
| Subject | Guatteria olivacea | en_US |
| Subject | Essential oil | en_US |
| Subject | Liver cancer | en_US |
| Subject | Antitumor | en_US |
| DeCS | Óleos Voláteis | en_US |
| DeCS | Neoplasias Hepáticas | en_US |
