| Author | Martins, Priscilla da Costa | |
| Author | Souza, Hugo Amorim dos Santos de | |
| Author | Blanco, Carolina Moreira | |
| Author | Oliveira, Luana Santos de | |
| Author | Pratt-Riccio, Lilian Rose | |
| Author | Ribeiro, Cláudio Tadeu Daniel | |
| Author | Totino, Paulo Renato Rivas | |
| Access date | 2022-09-27T19:51:46Z | |
| Available date | 2022-09-27T19:51:46Z | |
| Document date | 2022 | |
| Citation | MARTINS, Priscilla da Costa et al. Modulation of Signal Regulatory Protein (SIRP ) by Plasmodium Antigenic Extract: A Preliminary In Vitro Study on Peripheral Blood Mononuclear Cells. Microorganisms, v. 10, 903, p. 1 - 8, Apr. 2022. | en_US |
| ISSN | 2076-2607 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/54907 | |
| Language | eng | en_US |
| Publisher | MDPI | en_US |
| Rights | open access | |
| Subject in Portuguese | P. falciparum; P. vivax; LPS; Proteína reguladora de sinal/ Resposta imune inata | en_US |
| Title | Modulation of Signal Regulatory Protein (SIRP ) by Plasmodium Antigenic Extract: A Preliminary In Vitro Study on Peripheral Blood Mononuclear Cells | en_US |
| Type | Article | en_US |
| Abstract | Signal regulatory protein (SIRP ) is an immunoreceptor expressed in myeloid innate
immune cells that signals for inhibition of both phagocytosis and inflammatory response. Malaria
parasites have evolutionarily selected multiple mechanisms that allow them to evade host immune
defenses, including the modulation of cells belonging to innate immunity. Notwithstanding, little
attention has been given to SIRP in the context of immunosuppressive states induced by malaria.
The present study attempted to investigate if malaria parasites are endowed with the capacity of
modulating the expression of SIRP on cells of innate immune system. Human peripheral blood
mononuclear cells (PBMC) from healthy individuals were incubated in the presence of lipopolysaccharide
(LPS) or crude extracts of P. falciparum or P. vivax and then, the expression of SIRP was
evaluated by flow cytometry. As expected, LPS showed an inhibitory effect on the expression of
SIRP in the population of monocytes, characterized by cell morphology in flow cytometry analysis,
while Plasmodium extracts induced a significant positive modulation. Additional phenotyping
of cells revealed that the modulatory potential of Plasmodium antigens on SIRP expression was
restricted to the population of monocytes (CD14+CD11c+), as no effect on myeloid dendritic cells
(CD14CD11c+) was observed. We hypothesize that malaria parasites explore inhibitory signaling
of SIRP to suppress antiparasitic immune responses contributing to the establishment of infection.
Nevertheless, further studies are still required to better understand the role of SIRP modulation in
malaria immunity and pathogenesis. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malária. Rio de Janeiro, RJ, Brasil. | en_US |
| Subject | P. falciparum; P. vivax; LPS; innate immune response; Signal regulatory protein | en_US |
