| Author | Everall, Izzy | |
| Author | Nogueira, Christiane Lourenço | |
| Author | Bryant, Josephine M. | |
| Author | Sánchez-Busó, Leonor | |
| Author | Chimara, Erica | |
| Author | Duarte, Rafael da Silva | |
| Author | Ramos, Jesus Pais | |
| Author | Lima, Karla Valéria Batista | |
| Author | Lopes, Maria Luíza | |
| Author | Palaci, Moises | |
| Author | Kipnis, Andre | |
| Author | Monego, Fernanda | |
| Author | Floto, R. Andres | |
| Author | Parkhill, Julian | |
| Author | Leão, Sylvia Cardoso | |
| Author | Harris, Simon R. | |
| Access date | 2022-10-05T13:52:09Z | |
| Available date | 2022-10-05T13:52:09Z | |
| Document date | 2017 | |
| Citation | EVERALL, Izzy et al. Genomic epidemiology of a national outbreak of post-surgical Mycobacterium abscessus wound infections in Brazil. Microbial Genomics, v. 3, n. 5, p. 1-10, May 2017. | en_US |
| ISSN | 2057-5858 | |
| URI | https://www.arca.fiocruz.br/handle/icict/55000 | |
| Sponsorship | O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES), bolsa do Programa de Doutoradosanduíche no Exterior (PDSE). Processo nº 88881.187875/2018-01. | en_US |
| Language | eng | en_US |
| Publisher | Society for General Microbiology | |
| Rights | open access | en_US |
| Title | Genomic epidemiology of a national outbreak of post-surgical Mycobacterium abscessus wound infections in Brazil | en_US |
| Type | Article | en_US |
| DOI | 10.1099/mgen.0.000111 | |
| Abstract | An epidemic of post-surgical wound infections, caused by a non-tuberculous mycobacterium, has been on-going in Brazil. It has been unclear whether one or multiple lineages are responsible and whether their wide geographical distribution across Brazil is due to spread from a single point source or is the result of human-mediated transmission. 188 isolates, collected from nine Brazilian states, were whole genome sequenced and analysed using phylogenetic and comparative genomic approaches. The isolates from Brazil formed a single clade, which was estimated to have emerged in 2003. We observed temporal and geographic structure within the lineage that enabled us to infer the movement of sub-lineages across Brazil. The genome size of the Brazilian lineage was reduced relative to most strains in the three subspecies of Mycobacterium abscessus and contained a novel plasmid, pMAB02, in addition to the previously described pMAB01 plasmid. One lineage, which emerged just prior to the initial outbreak, is responsible for the epidemic of post-surgical wound infections in Brazil. Phylogenetic analysis indicates that multiple transmission events led to its spread. The presence of a novel plasmid and the reduced genome size suggest that the lineage has undergone adaptation to the surgical niche. | |
| Affilliation | Wellcome Trust Sanger Institute. Hinxton, Cambridge, UK. | en_US |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. | en_US |
| Affilliation | University of Cambridge. Department of Medicine. Molecular Immunity Unit. MRC Laboratory of Molecular Biology. Cambridge, UK. | |
| Affilliation | Wellcome Trust Sanger Institute. Hinxton, Cambridge, UK. | |
| Affilliation | Instituto Adolfo Lutz. Núcleo de Tuberculose e Micobacterioses. São Paulo, SP, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Centro de Referência Professor Helio Fraga. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Instituto Evandro Chagas. Seção de Bacteriologia e Micologia. Belém, PA, Brasil. | |
| Affilliation | Instituto Evandro Chagas. Seção de Bacteriologia e Micologia. Belém, PA, Brasil. | |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil. | |
| Affilliation | Universidade Federal de Goiás. Departamento de Microbiologia. Goiânia, GO, Brasil. | |
| Affilliation | Universidade Federal do Paraná. Departamento de Medicina Veterinária. Curitiba, PR, Brasil. | |
| Affilliation | University of Cambridge. Department of Medicine. Molecular Immunity Unit. MRC Laboratory of Molecular Biology. Cambridge, UK. | |
| Affilliation | Wellcome Trust Sanger Institute. Hinxton, Cambridge, UK. | |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. | |
| Affilliation | Wellcome Trust Sanger Institute. Hinxton, Cambridge, UK. | |
| Subject | Genomic epidemiology | |
| Subject | Mycobacterium abscessus | |
| Subject | Nosocomial infections | |
| Subject | Outbreak | |
| Subject | Transmission | |
| e-ISSN | 2057-5858 | |
