| Author | Mendes, Graziele | |
| Author | Baltazar, Ludmila de Matos | |
| Author | Souza, Daniele da Gloria de | |
| Author | Sá, Nivea Pereira de | |
| Author | Rosa, Luiz Henrique | |
| Author | Rosa, Carlos Augusto | |
| Author | Fagundes, Elaine Maria de Souza | |
| Author | Pereira, Jonas Ramos | |
| Author | Silva, Juliana Alves | |
| Author | Cota, Betania Barros | |
| Author | Johann, Susana | |
| Access date | 2022-10-13T13:46:00Z | |
| Available date | 2022-10-13T13:46:00Z | |
| Document date | 2018 | |
| Citation | MENDES, Graziele et al. Effects of cytochalasin E on Paracoccidioides brasiliensis. J Appl Microbiol., v. 125, n. 5, p. 1296-1307, 2018. doi: 10.1111/jam.14053. | en_US |
| ISSN | 1364-5072 | en_US |
| URI | https://www.arca.fiocruz.br/handle/icict/55122 | |
| Language | eng | en_US |
| Publisher | Blackwell Science | en_US |
| Rights | restricted access | |
| Title | Effects of cytochalasin E onParacoccidioides brasiliensis | en_US |
| Type | Article | |
| DOI | 10.1111/jam.14053 | |
| Abstract | AIMS: To determine the effects of cytochalasin E, isolated from the extremophile fungus Aspergillus felis, on the cells of Paracoccidioides brasiliensis Pb18.
METHODS AND RESULTS: Cytochalasin E showed a minimal inhibitory concentration of 3·6 μmol l-1 and minimum fungicidal concentration of 7·2 μmol l-1 on P. brasiliensis by in vitro microdilution and IC50 >964·0 μmol l-1 on murine macrophages. Its selectivity index (>263) indicated that this compound has selectivity for fungal cells. Morphological alterations were determined by optical and fluorescence microscopy, as well as scanning and transmission electron microscopy. Cytochalasin E affected P. brasiliensis bud-forming pseudohyphae, cell morphology, cell walls and cell membranes; caused the release of cellular material; and resulted in the production of reactive oxygen species. In murine macrophages, it affected cytoskeletal actin and inhibited phagocytosis.
CONCLUSION: Cytochalasin E may be useful as an antifungal prototype against P. brasiliensis and in studies on phagocytosis.
SIGNIFICANCE AND IMPACT OF THE STUDY: Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM). Treatment is prolonged to control the clinical manifestations and prevent relapse. The study on the effects of cytochalasin E in P. brasiliensis is important because it can be used as a prototype for new antifungal drugs and consequently, broadens the treatment options for PCM. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Química de Produtos Naturais Bioativos. Belo Horizonte, MG, Brazil. | en_US |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
| Subject | Paracoccidioides brasiliensis | en_US |
| Subject | antifungal activity | en_US |
| Subject | cell membrane | en_US |
| Subject | cell wall | en_US |
| Subject | cytochalasin E | en_US |
| Subject | reactive oxygen species | en_US |
